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Acta Parasitologica

, Volume 64, Issue 1, pp 13–18 | Cite as

Molecular Detection of Zoonotic Microsporidia in Domestic Cats in Turkey: A Preliminary Study

  • Didem PekmezciEmail author
  • Gokmen Zafer Pekmezci
  • Alparslan Yildirim
  • Onder Duzlu
  • Abdullah Inci
Article
  • 18 Downloads

Abstract

Objective

This preliminary study was conducted to reveal that the molecular identity of Encephalitozoon spp. and Enterocytozoon bieneusi in indoor domestic cats’ fecal samples from Turkey was screened using the PCR.

Materials and Methods

Nested PCR was performed using MSP and EBITS primers. All of the amplification products were sequenced to identify the microsporidia species.

Results

Four (5.5%) and three (4.1%) genomic DNA isolates of the fecal samples from 72 indoor domestic cats showed amplification of the ITS regions of E. bieneusi and Encephalitozoon spp., respectively. Two different genotypes, D and IV, of E. bieneusi were determined in two cats each based on the ITS sequence analyses. Moreover, Encephalitozoon spp. sequence analyses revealed three isolates belonging to E. intestinalis.

Conclusions

This preliminary study has provided the first molecular data on the zoonotic genotypes of E. bieneusi and E. intestinalis in cats in Turkey. Furthermore, E. bieneusi genotype IV (accession number MG727664) was submitted to GenBank for the first time in the Western Palearctic Region as hosted by a domestic cat. Additionally, E. intestinalis (accession number MG570080) was also submitted to GenBank as a valid ITS sequence for the first time as hosted by a domestic cat worldwide.

Keywords

Molecular detection Encephalitozoon intestinalis Enterocytozoon bieneusi Cat Turkey 

Notes

Acknowledgements

We are appreciated to our clients for their special effort and thank to all of them for collecting the study materials from their lovely cats.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • Didem Pekmezci
    • 1
    Email author
  • Gokmen Zafer Pekmezci
    • 2
  • Alparslan Yildirim
    • 3
  • Onder Duzlu
    • 3
  • Abdullah Inci
    • 3
  1. 1.Department of Internal Medicine, Faculty of Veterinary MedicineUniversity of Ondokuz MayisSamsunTurkey
  2. 2.Preclinical Sciences, Faculty of Veterinary MedicineUniversity of Ondokuz MayisSamsunTurkey
  3. 3.Department of Parasitology, Faculty of Veterinary MedicineUniversity of ErciyesKayseriTurkey

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