Plasminogen Activators and Tiaprofenic Acid in Inflammation
Treatment with tiaprofenic acid appreciably reduced the level of plasminogen activators in the medium of 3T3-Balb mouse flbroblasts, as revealed by both a fibrin plate assay and amidolytic determination with chromogenic substrates. At the same time, tiaprofenic acid was able to inhibit the production of plasminogen activators induced by phorbol myristate acetate, a powerful inflammation and tumour promoter, added to the cell monolayers. By isolating the inhibitors of plasminogen activators it was possible to show that the decrease of fibrinolytic activity produced by tiaprofenic acid is not related to an increase of inhibitors. Rather, a decrease of activators seems to take place.
Synovial fluid samples from 4 patients before and after treatment with tiaprofenic acid were also assayed for plasminogen activator activity by the fibrin lysis method. In 3 of the 4 cases a marked decrease after treatment was evident. The one unresponsive patient suffered from a para-neoplastic arthritis.
KeywordsPlasminogen Activator Synovial Fluid Fibrinolytic Activity Tiaprofenic Acid Synovial Fluid Sample
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- Christman JK, Silverstein SG, Acs G. Plasminogen activators. In Barret (Ed.) Research monographs in cell and tissue physiology II. Proteinases in mammalian cells and tissues, pp. 90–149, Elsevier/North-Holland, Amsterdam, 1977Google Scholar
- Fibbi G, Del Rosso M, Pasquali F, Serni U, Chiarugi V. Control of proteolysis and lipolysis by the non-steroid anti-inflammatory drug methoxynaphthyl-propionate (naproxen). Xth European Congress of Rheumatology, Moscow, pp. 18–24, June 28, 1983Google Scholar
- Hamilton JA, Slywka J. Stimulation of human synovial fibroblast plasminogen activator production by mononuclear cell supernatants. Journal of Immunology 126: 851–855, 1981Google Scholar
- Nilsson IM, Hedner U, Pandolfi M. The measurement of fibrinolytic activities. In Markwardt (Ed.) Handbook of experimental pharmacology, Springer, Berlin, 1978Google Scholar
- Reich E, Rifkin DB, Shaw E. (Eds). Proteases and biological control, Cold Spring Harbor Conference on Cell Proliferation, Vol. 2, 1975Google Scholar