Clinical Pharmacokinetics

, Volume 9, Supplement 1, pp 49–60 | Cite as

Ultrafiltration vs Equilibrium Dialysis for Determination of Free Fraction

  • William F. Bowers
  • Scott Fulton
  • Judith Thompson
Article

Summary

The analytical basis of membrane separation in determining plasma free drug concentrations is developed from consideration of the generalised ideal mass action and conservation laws applied to thermodynamic states of differing component volumes. The intuitively surprising theoretical independence of free drug concentration with changing fractional volume of protein predicts the equivalence of final free drug concentration after uniform pre-dilution with that after equilibrium dialysis against the same volume of buffer. Similarly, the ultrafiltrate free concentration of drugs in ideal plasma binding equilibrium is theoretically constant, regardless of the extent of reduction in fractional volume of binding protein during filtration.

The pitfalls in calculating free and bound fraction and concentration from equilibrium dialysis are reviewed and expressions presented to correct the major artefacts. The dilution behaviour of drugs is predicted from the normalised mass action model. Practical aspects of validating ultrafiltration free drug levels using equilibrium dialysis are outlined.

Keywords

Free Drug Disopyramide Free Fraction Equilibrium Dialysis Free Concentration 

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Copyright information

© ADIS Press Limited. 1984

Authors and Affiliations

  • William F. Bowers
    • 1
  • Scott Fulton
    • 1
  • Judith Thompson
    • 1
  1. 1.Amicon CorporationDanversUSA

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