Liver Transplantation for Acute Intermittent Porphyria: Biochemical and Pathologic Studies of the Explanted Liver
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Acute intermittent porphyria (AIP) is an autosomal-dominant hepatic disorder caused by the half-normal activity of hydroxymethylbilane (HMB) synthase. Symptomatic individuals experience life-threatening acute neurovisceral attacks that are precipitated by factors that induce the hepatic expression of 5-aminolevulinic acid synthase 1 (ALAS1), resulting in the marked accumulation of the putative neurotoxic porphyrin precursors 5-aminolevulinic acid (ALA) and porphobilinogen (PBG). Here, we provide the first detailed description of the biochemical and pathologic alterations in the explanted liver of an AIP patient who underwent orthotopic liver transplantation (OLT) due to untreatable and debilitating chronic attacks. After OLT, the recipient’s plasma and urinary ALA and PBG rapidly normalized, and her attacks immediately stopped. In the explanted liver, (a) ALAS1 mRNA and activity were elevated approximately ∼3- and 5-fold, and ALA and PBG concentrations were increased ∼3- and 1,760-fold, respectively; (b) uroporphyrin III concentration was elevated; (c) microsomal heme content was sufficient, and representative cytochrome P450 activities were essentially normal; (d) HMB synthase activity was approximately half-normal (∼42%); (e) iron concentration was slightly elevated; and (f) heme oxygenase I mRNA was increased approximately three-fold. Notable pathologic findings included nodular regenerative hyperplasia, previously not reported in AIP livers, and minimal iron deposition, despite the large number of hemin infusions received before OLT. These findings suggest that the neurovisceral symptoms of AIP are not associated with generalized hepatic heme deficiency and support the neurotoxicity of ALA and/or PBG. Additionally, they indicate that substrate inhibition of hepatic HMB synthase activity by PBG is not a pathogenic mechanism in acute attacks.
Acute intermittent porphyria (AIP), the most common acute hepatic porphyria, is an autosomal-dominant disorder due to the half-normal activity of the heme biosynthetic enzyme, hydroxymethylbilane (HMB) synthase (1). Symptomatic heterozygotes, most (∼90%) of which are women, experience episodic life-threatening acute neurovisceral attacks that typically begin with severe abdominal pain and may include hypertension, tachycardia, constipation, motor weakness and seizures. These attacks are precipitated by certain drugs, dieting and hormonal factors that increase the hepatic expression of 5-aminolevulinic acid synthase 1 (ALAS1) (1). When hepatic ALAS1 is induced, the half-normal activity of HMB synthase becomes rate-limiting, leading to decreased heme biosynthesis and depletion of the hepatic “free” heme pool. Depletion of the “free” heme pool leads to further induction of hepatic ALAS1 expression and increased production and accumulation of the porphyrin precursors aminolevulinic acid (ALA) and porphobilinogen (PBG) (1).
The current approved treatment for the acute attacks is intravenous hemin infusions, which provide sufficient “free” heme to downregulate hepatic ALAS1 activity, leading to decreased production of ALA and PBG. Patients suffering recurrent attacks (particularly women who have cyclic attacks with their menstrual cycles) may be treated prophylactically with weekly to monthly hemin infusions and/or gonadotropin-releasing hormone analogs to prevent ovulation. However, recurrent attacks are often difficult to control and may result in chronic symptoms, particularly chronic neuropathic pain and progressive neuropathy that markedly impair the quality of life (1). Recently, orthotopic liver transplantation (OLT) was performed on several AIP patients in Europe who failed medical management for their recurrent severe attacks (2, 3, 4, 5, 6). Patients who were successfully transplanted normalized their urinary ALA and PBG levels and have remained attack-free up to 10 years after transplantation (3,5).
Two major hypotheses regarding the pathobiology of the acute attacks have been proposed: first, the hepatic metabolites ALA and/or PBG are neurotoxic and, second, heme deficiency leads to decreased neuronal hemoprotein production and causes the acute neurovisceral symptoms (7). The success of OLT in treating these severely affected AIP patients demonstrated that the liver is the major etiologic site of pathology and strongly supports the hypothesis that the markedly increased levels of ALA and/or PBG are the primary mediators of the acute attacks. In further support of this concept, when the explanted livers of AIP patients were transplanted into nonporphyric recipients with hepatocellular carcinoma (that is, “domino” liver transplants), recipients developed acute porphyric attacks characterized by increased urinary ALA and PBG levels (8). However, the contribution of generalized heme deficiency to disease pathogenesis is not entirely clear, since hemoprotein function in human AIP tissues during an acute attack have not been reported.
Here we describe the clinical and biochemical effects of the first OLT in the Americas for a severely affected AIP patient and document in detail the relevant biochemical and pathologic abnormalities in the explanted porphyric liver. Unlike previous reports in which only daily urinary ALA or PBG levels were obtained, plasma and urinary ALA and PBG concentrations were monitored frequently after transplantation to determine the kinetics of the toxic metabolite clearance. The data presented are the first from the liver of an AIP patient with debilitating and continuous symptoms, and they provide evidence that the hepatic overproduction and neurotoxic effects of ALA and/or PBG account for the neurovisceral manifestations.
Materials and Methods
Collection of Human Tissues and Body Fluids
Small pieces of the explanted AIP liver (∼5 g) were snap-frozen in liquid nitrogen and stored in the dark at −80°C until use. Control liver tissues were obtained through the Liver Tissue Cell Distribution Center at the University of Minnesota. Plasma and urine samples were immediately frozen and stored in the dark at −80°C until use. These studies were made exempt by the Icahn School of Medicine at Mount Sinai Institutional Review Board.
Porphyrin Precursor and Porphyrin Isomer Quantitation
Liver tissues were homogenized in 3 v/w of chilled 50 mmol/L HEPES buffer, pH 7.5, containing 1 mmol/L EDTA and 150 mmol/L KCl and then centrifuged at 10,000g for 10 min at 4°C. The supernatant was placed on a VWR centrifugal filter and centrifuged at 10,000g for 10 min at 4°C. Hepatic, plasma and urinary ALA and PBG concentrations were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS), as previously described (9,10).
For porphyrin analyses, liver homogenates were deproteinized in 20% TCA/DMSO (1:1, v/v), centrifuged for 10 min at 10,000g, and the porphyrins in the supernatants were analyzed by ultraperformance liquid chromatography (ACQUITY UPLC®, Waters Corporation), as previously described (11).
ALAS1 and HMB Synthase Enzyme Activity Assays
ALAS1 and HMB synthase activities were determined in homogenized livers as previously described (9,11). Uroporphyrin fluorescence was measured in a Foci Farrand Spectrofluorometer System 3 (Farrand Optical Components and Instruments).
Microsomal Heme Content and Cytochrome P450 (CYP) Activity Assays
Liver microsomes were isolated as previously described (12), and total protein content of the purified microsomes was determined by using the Bio-Rad DC Protein Assay Kit. Heme content was determined by using the pyridine hemochrome method of Berry and Trumpower (13). CYP2E1 activity was determined as described (12), whereas CYP1A2 activity was measured by using the P450-Glo CYP1A2 Assay kit (Promega) according to the manufacturer’s instructions. Combined activity of CYP1A1, CYP1B1 and CYP3A7 was determined using the Promega P450-Glo CYP1A1/CYP1B1 Assay kit, which uses luciferin-6′ chloroethyl ether as a substrate.
Hepatic Iron Quantitation
Liver samples were dried in an 80°C oven until a constant weight was attained on two consecutive readings and then digested by using 30% high-purity hydrogen peroxide (GFS Chemicals), as previously described (14). The digested tissues were dissolved in 1% ultra-pure nitric acid (Sigma-Aldrich), and iron concentrations were determined by graphite furnace-atomic absorption spectrophotometry (GF-AAS) by using a Varian Instruments Model-240Z Zeeman atomic absorption spectrophotometer (Varian, Inc.) equipped with a Varian GTA-120 graphite tube analyzer, a PSD-120 programmable sample dispenser and a Varian UltrAA high-intensity boosted hollow cathode lamp. The instrument and furnace settings used are provided in Supplementary Table S1. Data acquisition was carried out with the Varian SpectrAA software, and hepatic iron content was expressed as µg/mg dry tissue weight. To minimize external metal contaminations, all procedures were performed in a clean environment and auto-sampler cups were treated with 1% ultra-pure nitric acid and washed with ddH2O before use.
RNA Extraction and Real-Time PCR Quantification
RNA isolation, reverse transcription and real-time polymerase chain reaction (PCR) were performed as previously described (9), by using predesigned Taqman® assays (Life Technologies) for ALAS1 (assay ID: Hs00963534_m1), HO-1 (Hs01110251_m1), interleukin (IL)-1β (IL-1β; Hs01555410_m1), IL-6 (IL-6; Hs00985639_m1), tumor necrosis factor α (TNFα; Hs01113624_g1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Hs02758991_g1). Relative ALAS1, HO-1, IL-1β, IL-6 and TNFα transcript levels were determined by the comparative Ct method using GAPDH as an internal control. To validate the Taqman assay for HO-1, Huh-7 cells were cultured in Dulbecco modified Eagle medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin, plated into six-well plates, and incubated for 24 h with or without 10 µmol/L hemin (Sigma-Aldrich), a potent inducer of HO-1 expression. Relative HO-1 mRNA levels were determined, and increased HO-1 expression was confirmed in the hemin-treated cells.
Western Blot Analyses
ALAS1 Western blot analysis was performed as previously described (9), with the following modifications. Liver samples were homogenized in chilled lysis buffer (20 mmol/L HEPES, 150 mmol/L sodium chloride, pH 7.4, 0.2% IGEPAL, 0.5% Triton X-100 and protease inhibitor cocktail [Sigma-Aldrich]), and protein concentrations were determined using the Bio-Rad Protein Assay Dye Reagent Concentrate kit. After separation and transfer of the proteins, the polyvinylidene difluoride membranes were blocked with 5% nonfat dry milk in phosphate-buffered saline with 0.1% Tween-20 for 1 h at room temperature, incubated with anti-ALAS1 antibody (1:2,000 dilution; Fitzgerald Industries International) for 1 h at room temperature and were then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG antibody (1:20,000 dilution; Santa Cruz Biotechnology) for 1 h at room temperature. Signals were detected using Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore, Billerica, MA, USA). The membranes were re-probed with anti-GAPDH-HRP antibody (Santa Cruz Biotechnology) to normalize loading amounts.
Pathologic Examination of Explanted Liver
Representative liver tissues were dissected and fixed in either 10% phosphate-buffered formalin for light microscopy or 3% glutaraldehyde in 0.2 mol/L sodium cacodylate buffer for electron microscopy. Formalin-fixed livers were embedded in paraffin, cut into 4–6-µm sections and stained with hematoxylin and eosin, Masson trichrome or Perls stain. Images were captured by a Nikon DS-L1 Camera Control Unit (Nikon Corporation) attached to a Nikon Eclipse Ci light microscope (Nikon Corporation). Liver tissues fixed in glutaraldehyde were embedded in Embed 812 and cut into ultra-thin sections, stained with uranyl acetate and lead citrate and then observed on a Hitachi H-7650 transmission electron microscope.
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A 36-year-old Caucasian woman diagnosed with AIP at age 27 years (HMBS gene mutation, c.517C>T [p.Arg173Trp]) had onset of recurrent and debilitating acute attacks at age 25 years. She developed complications, including chronic axonal polyneuropathy and motor paresis. During the 5-year period before transplantation, she received a total of 267 hemin (Panhematin®) infusions (73,743 mg hemin, corresponding to 6,320 mg iron), of which 116 infusions (35,993 mg hemin, corresponding to 3,232 mg iron) were administered during the 18 months before OLT. Her course was complicated by multiple infusion port infections. She became increasingly disabled and depressed and developed chronic abdominal, back and lower-extremity pain that required continued high doses of opioid analgesics, including hydromorphone and fentanyl. Because the patient’s symptoms became completely refractory to hemin, the infusions were discontinued 12 wks before transplantation. She was unable to work or to complete her college education because of continuous symptoms. After 13 months of nearly continuous hospitalization, OLT was considered.
Because AIP does not involve impairment of vital liver functions, conventional scoring systems for organ allocation were not applicable. Her case was reviewed by the regional review board for allocation of priority point exceptions. An initial model for end-stage liver disease (MELD) score of 25 was requested, with the plan to apply for higher priority points every 90 d if the patient’s clinical condition did not improve. Over the following 3 months, the patient’s condition worsened, requiring her to remain hospitalized for symptom management, including intravenous analgesia and antiemetics. After serial appeals to the regional review board, a final priority MELD score of 35 was obtained and transplantation was performed. The patient received a cadaveric liver from a 49-year-old male donor who died from a stroke. The cold ischemia time was 4.5 h.
On the basis of previous reports of a high incidence of hepatic arterial thrombosis after OLT in AIP patients (3), anticoagulation therapy was initiated immediately after transplantation. No complications occurred during or after the surgical procedure, and transfusions from the blood bank were unnecessary. The debilitating abdominal pain resolved immediately after transplantation, and her quality of life improved significantly. She was treated with immunosuppressive therapy including tacrolimus, prednisone and mycophenolate and was maintained on tacrolimus after mycophenolate and prednisone were tapered and discontinued at 9 and 14 months posttransplantation, respectively. She resumed full daily activities and returned to college 4 months posttransplantation. At 24 months posttransplantation, the patient continued to do well and had no acute attacks. Her neuropathic bilateral leg pain markedly improved, and although her mid-back pain attributed to porphyric nerve damage persisted, she continued to function well with multimodal pain treatment, including low-dose chronic opioid therapy. Her weight before OLT had increased to 92.9 kg (BMI 38.9 kg/m2) because of prolonged hospitalizations and dextrose infusions. One year after transplantation, she reduced her weight to 55.2 kg (BMI 22.9 kg/m2).
Pre- and Posttransplantation Porphyrin Precursor Studies
Biochemical Studies of the AIP Liver
HMB synthase activities in AIP and control livers.
HMB synthase activity (nmol/h/mg protein)
Control (n = 5)
5.9 ± 2.4 (3.1–8.1)
AIP (n = 1)
2.5 ± 0.6 (1.6–3.0)
Pathological Studies of the AIP Liver
Porphyrin precursor and porphryin levels in AIP and control livers.
Controls (n = 5)
AIP (n = 1)
Fold-change over controls
20 ± 4.6
58 ± 5.1
2.5 ± 1.7
4,442 ± 162
12 ± 6.6
8.1 ± 13
0.7 ± 1.1
13 ± 12
Microsomal heme content and CYP activities in AIP and control livers.
Controls (n = 5)
AIP (n = 1)
Microsomal heme content
0.80 ± 0.26
1.47 ± 0.27
105 ± 20
116 ± 2.8
1.26 ± 0.30
1.10 ± 0.09
CYP1A1 + CYP1B1 + CYP3A7
6,049 ± 309
4,498 ± 15
Iron content in AIP and control livers.
Hepatic iron content (µg iron/mg dry liver)
Control (n = 5)
0.53 ± 0.21 (0.25–0.77)
AIP (n = 1)
Light microscopy. Histologically, representative sections were heterogeneous. Most portal areas showed mild nonspecific lymphocytic inflammation and mild ductular reaction (Figure 3C). While bile ducts were preserved, portal vein branches were either partially or completely obliterated or dystrophic, as characterized by herniation of portal vein branches into the periportal spaces. Although the parenchyma appeared nodular, the absence of established cirrhosis was confirmed by a Masson trichrome stain. The majority of nodules were composed of hyperplastic hepatocytes, and the periphery of these nodules consisted of atrophic plates (Figure 3C). However, some sections taken from close to the hilum showed incomplete nodules consisting of atrophic parenchyma that were partially or completely surrounded by delicate bridging fibrous septa (Figure 3D). Most of the fibrosis occurred in the portal areas. The hepatic parenchyma had a brownish appearance at low power, due to coarse brown intracytoplasmic granules that were most prominent in centrilobular hepatocytes (Figure 3E). This pigment resembled lipofuscin, although slightly coarser in texture. Also seen was patchy steatosis ranging from focal to moderate in degree (Figure 3F). The steatotic vacuoles were mainly small droplets admixed with a few medium-sized droplets. Features of steatohepatitis were absent. Minimal iron deposition was detected by Perls stain. The main findings were nodular regenerative hyperplasia (NRH) with features of focal incomplete septal cirrhosis, mild steatosis and lipofuscin pigment deposition.
Electron microscopy. Ultrastructurally, the liver had a relatively normal histoarchitecture with generally normal peroxisomes, endoplasmic reticulum and mitochondria, although some focal hepatocytes had enlarged mitochondria. There was some cytoplasmic neutral lipid in hepatocytes and an occasional crystalline structure resembling cholesterol crystals. Kupffer cells, and possibly endothelial cells, had many electrondense lysosomal granules. The majority of these granules were lamellar, corresponding to lipofuscin, whereas a much smaller number were densely granular, consistent with iron deposition.
It is estimated that approximately 10% of AIP heterozygotes develop acute porphyric attacks (1). Of these individuals, ∼90% are women who have cyclic attacks during the luteal phase of their menstrual cycles or even more frequent attacks (1,7,16). Certain HMBS genotypes were found to be more frequent among AIP heterozygotes who have recurrent attacks, including c.517C>T (p.Arg173Trp), c.518G>A (p.Arg173Gln) and c.673C>T (p.Arg225Ter) (17). Here, we report a woman with the HMBS p.Arg173Trp mutation whose recurrent and debilitating attacks became refractory to hemin infusions, leading to OLT. After OLT, the recurrent acute attacks stopped immediately and have not recurred for over 24 months. Moreover, her abdominal pain resolved rapidly and her peripheral neuropathy significantly improved. Her plasma and urinary ALA and PBG concentrations normalized by 5 and 31 h posttransplantation (Figures 1A, B), respectively, more rapidly than previously reported. The slower clearance of the elevated PBG relative to ALA presumably reflected the fact that pretransplantation plasma and urinary PBG levels were markedly more elevated (940- to 1,180-fold and 13- to 69-fold over normal values, respectively) than the ALA levels (10- to 14-fold and 1.5- to 6-fold over normal, respectively).
Of particular note were the biochemical and pathologic studies of the explanted porphyric liver. Hepatic ALAS1 expression was increased (Figures 2A–C), consistent with the increased levels of ALA and PBG. Notably, hepatic ALA was only moderately elevated (∼3-fold) compared with the dramatically elevated hepatic PBG levels (∼1,780-fold; Table 2), indicating that ALA overproduced in the liver was either rapidly released into the circulation for excretion or metabolized to PBG by the abundant hepatic ALA-dehydratase activity. HMB synthase activity was ∼42% compared with the mean activity in control livers (Table 1), consistent with the previous finding that prokaryotic expression of the p.Arg173Trp HMBS allele resulted in <1% residual HMB synthase activity (18). That the HMB synthase activity was approximately half-normal argued against the previously proposed hypothesis that the elevated PBG may significantly decrease the half-normal HMB synthase activity during an acute attack via substrate inhibition (7). The hepatic uroporphyrin III concentration was elevated approximately three-fold over the mean value in control livers, indicating that sufficient PBG was converted to hydroxymethylbilane despite the half-normal HMB synthase activity (Table 2). Moreover, microsomal heme content was increased compared with the mean level in the control livers, and the catalytic activities of several CYP450 enzymes (CYP1A2, CYP2E1, and CYP1A1 + CYP1B1 + CYP3A7) were within the normal range in the AIP liver (Table 3 and Supplementary Figure S1). Thus, abdominal pain (the most common acute attack symptom) occurred in the presence of increased hepatic ALAS1 expression and markedly elevated hepatic and circulating ALA and PBG levels, but in the absence of generalized hepatic heme deficiency. These findings suggest that the hepatic heme deficiency in AIP is confined to depletion of the “free” heme pool, leading to greater induction of ALAS1 and accumulation of the putatively neurotoxic ALA and/or PBG. Importantly, these findings validate the use of a liver-directed RNA interference (RNAi) therapeutic targeting hepatic ALAS1 mRNA for the prevention and treatment of acute attacks, as shown in recent preclinical studies (9).
The expression of HO-1, the first and rate-limiting enzyme in heme catabolism, also was induced in the AIP liver (Figure 2D). HO-1 can be induced at the transcriptional level by heme accumulation, as well as by various cellular stresses, including inflammatory cytokines, hypoxia and oxidative stress (19). Increased HO-1 activity has antioxidant and antiinflammatory effects and plays a cytoprotective role under stress conditions (19). Our transplant recipient did not receive therapeutic hemin for 3 months before OLT, thereby excluding exogenous heme as a mechanism of HO-1 induction. As microsomal heme content was elevated in the AIP liver relative to mean control levels (Table 3), it is possible that hepatic HO-1 expression was induced as a result of increased endogenous heme production. Alternatively, HO-1 expression may have been elevated as a result of the chronic nonspecific portal inflammation and/or incomplete septal cirrhosis, as evidenced by the explant’s pathological examination (Figures 3C, D). Indeed, the mRNA expression of proinflammatory cytokines IL-1β, IL-6 and TNFα were elevated in the AIP liver compared with mean control levels (Supplementary Table S2). The upregulated HO-1 activity may have exacerbated porphyric symptoms, as the increased heme degradation may contribute to elevated levels of the putatively neurotoxic ALA and PBG by increasing the transcriptional activity of ALAS1 (20,21) and the import of precursor ALAS1 into the mitochondria, where the protein is converted to its mature form (22).
To date, the few studies of AIP liver pathology describe nonspecific findings, including minimal to moderate steatosis, hemosiderosis and lipofuscinosis (3,23, 24, 25, 26, 27, 28). None of the previously reported cases described the NRH or incomplete septal cirrhosis seen in our patient (Figures 3C, D). NRH and incomplete septal cirrhosis both involve vascular obstruction of small hepatic arteries or portal branches and may progress to noncirrhotic portal hypertension. NRH is typically associated with systemic autoimmune diseases, hematological disorders and various drugs, particularly immunosuppressant and chemotherapeutic agents (29,30), whereas incomplete septal cirrhosis has been described in patients with viral hepatitis, alcoholism and exposure to vasculotoxic chemicals such as arsenic (31). Our patient did not have any of these conditions, nor was she on medication known to induce NRH or incomplete septal cirrhosis. It is possible that AIP itself may have led to these pathologic changes, although there is no known association between AIP and hepatic vascular injury or an intrahepatic hypercoaguable state. While it is uncommon for NRH, characterized by a lack of fibrosis, and incomplete septal cirrhosis to coexist within the same liver, such cases have been reported (32).
Also notable was the observation that hepatic iron deposition was minimal in the AIP liver, despite the remarkable number of hemin infusions during the 5 years pretransplantation. In line with this finding, the patient’s hepatic iron content was only mildly elevated (∼5-fold) compared with the control livers (Table 4), and the patient’s ferritin did not exceed 760 ng/mL, even during the time she was receiving frequent hemin infusions. These findings suggest that hemin-induced iron overload most likely was not a contributing mechanism to the hepatic architectural changes, including NRH and fibrosis.
These studies document the first OLT in the Americas in a severely affected AIP patient that resulted in the rapid clearance of the accumulated ALA and PBG, prevented further acute attacks and ameliorated chronic neuropathic pain. Biochemical studies of the explanted AIP liver provided important insights into the disease pathogenesis, indicating that (a) the acute and persistent neurovisceral pain in AIP is most likely associated with depletion of the hepatic “free” heme pool, but not with generalized hepatic heme deficiency, and (b) substrate inhibition of hepatic HMB synthase activity by PBG is not a likely pathogenic mechanism in the acute attacks. Although OLT is potentially curative, it is associated with high morbidity and limited organ availability and should be reserved as a last resort therapy for AIP patients with intractable, severe and frequent neurovisceral manifestations.
Future novel therapeutic approaches that are safe and effective are desirable for the prevention and treatment of the acute porphyric attacks.
M Yasuda and RJ Desnick received research support from Alnylam Pharmaceuticals and are inventors on intellectual property licensed through the Icahn School of Medicine at Mount Sinai to Alnylam Pharmaceuticals. RJ Desnick is a consultant for Recordati Rare Diseases and Alnylam Pharmaceuticals. KE Anderson is a consultant for Mitsubishi Tanabe Pharma America.
We thank Ethellyn Panta (Icahn School of Medicine at Mount Sinai) and Hector Bergonia (University of Utah School of Medicine) for their excellent technical assistance. This work was supported in part by Career Development Awards K01 DK087971 (to M Yasuda) and K23 DK095946 (to M Balwani) from the National Institutes of Health (NIH) and a cooperative grant (U54 DK083909) for the Porphyrias Consortium, which is a part of the NIH Rare Diseases Research Network (RDCRN) and supported through collaboration between the NIH Office of Rare Diseases Research (ORDR) at the National Center for Advancing Translational Science (NCATS) and National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. The Liver Tissue Cell Distribution System at the University of Minnesota provided the control human liver tissues and is funded by NIH contract HHSN276201200017C.
- 1.Anderson KE, Sassa S, Bishop DF, Desnick RJ. (2001) Disorders of heme biosynthesis: X-linked sideroblastic anemia and the porphyrias. 8th edition. 8th edition. In: The Metabolic and Molecular Bases of Inherited Disease. Scriver CR, Beaudet AL, Sly WS, Valle D, Childs B, Kinzler KW, Vogelstein B (eds.) McGraw-Hill, New York, pp. 2961–3062.Google Scholar
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