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Molecular Medicine

, Volume 21, Issue 1, pp 233–241 | Cite as

Loss of Allograft Inflammatory Factor-1 Ameliorates Experimental Autoimmune Encephalomyelitis by Limiting Encephalitogenic CD4 T-Cell Expansion

  • Prameladevi Chinnasamy
  • Sarah E. Lutz
  • Dario F. Riascos-Bernal
  • Venkatesh Jeganathan
  • Isabel Casimiro
  • Celia F. Brosnan
  • Nicholas E. S. Sibinga
Research Article

Abstract

Experimental autoimmune encephalomyelitis (EAE), an animal model of human multiple sclerosis (MS), is mediated by myelinspecific autoreactive T cells that cause inflammation and demyelination in the central nervous system (CNS), with significant contributions from activated microglia and macrophages. The molecular bases for expansion and activation of these cells, plus trafficking to the CNS for peripheral cells, are not fully understood. Allograft inflammatory factor-1 (Aif-1) (also known as ionized Ca2+ binding adapter-1 [Iba-1]) is induced in leukocytes in MS and EAE; here we provide the first assessment of Aif-1 function in this setting. After myelin oligodendrocyte glycoprotein peptide (MOG35–55) immunization, Aif-1-deficient mice were less likely than controls to develop EAE and had less CNS leukocyte infiltration and demyelination; their spinal cords contained fewer CD4 T cells and microglia and more CD8 T cells. These mice also showed significantly less splenic CD4 T-cell expansion and activation, plus decreased proinflammatory cytokine expression. These findings identify Aif-1 as a potent molecule that promotes expansion and activation of CD4 T cells, plus elaboration of a proinflammatory cytokine milieu, in MOG35–55-induced EAE and as a potential therapeutic target in MS.

Notes

Acknowledgments

We thank the staff of the Albert Einstein College of Medicine Analytical Imaging Facility, FACS and Histology core facilities of Albert Einstein College of Medicine for their expert assistance. This work was supported in part by Pilot Project PP1500 from the National Multiple Sclerosis Foundation and by grant HL67944 from the National Institutes of Health.

Supplementary material

10020_2015_2101233_MOESM1_ESM.pdf (1.1 mb)
Supplementary material, approximately 1.13 MB.

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Authors and Affiliations

  • Prameladevi Chinnasamy
    • 1
    • 2
  • Sarah E. Lutz
    • 3
  • Dario F. Riascos-Bernal
    • 1
    • 2
  • Venkatesh Jeganathan
    • 4
  • Isabel Casimiro
    • 1
    • 2
  • Celia F. Brosnan
    • 3
  • Nicholas E. S. Sibinga
    • 1
    • 2
    • 5
  1. 1.Department of Medicine (Cardiovascular Division)Albert Einstein College of Medicine, Forchheimer G46BronxUSA
  2. 2.Department of Developmental and Molecular BiologyAlbert Einstein College of MedicineBronxUSA
  3. 3.Department of NeuroscienceAlbert Einstein College of MedicineBronxUSA
  4. 4.Center for Autoimmune and Musculoskeletal DiseaseThe Feinstein Institute of Medical ResearchManhassetUSA
  5. 5.Wilf Family Cardiovascular Research InstituteAlbert Einstein College of MedicineBronxUSA

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