Molecular Medicine

, Volume 17, Issue 11–12, pp 1131–1131 | Cite as

Comment on “Tissue Factor-Dependent Chemokine Production Aggravates Experimental Colitis”

  • Julia E. Gambone
  • A. Phillip OwensIII
  • Nigel MackmanEmail author
Open Access
Letters to the Editor

The article by Queiroz et al. in the September-October 2011 issue of Molecular Medicine (1) uses a genetic approach to examine the role of tissue factor (TF) in colitis. The authors conclude that TF contributes to colitis by enhancing expression of the granulocyte chemoattractant keratinocyte-derived chemokine (KC), leading to granulocyte recruitment. Three experimental groups of mice are used: C57BL/6, TF+/−/hTF+ (50% mice) and TF−/−/hTF+ (1% mice). The 50% and 1% mice generated by our laboratory (2) are on a genetic background that is not fully C57BL/6. These mice were created by backcrossing six generations with C57BL/6 mice. However, an increase in spontaneous hemorrhage caused us to discontinue this backcrossing (3). We therefore concluded that the 50% and 1% mice are not on a pure C57BL/6 genetic background. Many studies have shown significant differences in the phenotypes between different strains of mice (4,5). Subsequently, drawing conclusions regarding disease mechanisms when data are collected from different strains rather than comparing littermates is difficult. The correct control population for these studies should consist of TF+/+/hTF+ mice, generated by intercrossing TF+/−/hTF+ mice. Importantly, many of the differences reported by Queiroz et al. (1) may reflect differences in strain (C57BL/6 versus either the 50% or 1% mice) rather than differences in TF expression (50% versus 1% mice). Therefore, the data presented in this paper should be interpreted with caution.


The authors declare that they have no competing interests as defined by Molecular Medicine, or other interests that might be perceived to influence the results and discussion reported in this paper.


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Copyright information

© The Feinstein Institute for Medical Research 2011

Authors and Affiliations

  • Julia E. Gambone
    • 1
  • A. Phillip OwensIII
    • 2
  • Nigel Mackman
    • 2
    Email author
  1. 1.Department of Pathology, Division of Hematology and Oncology, McAllister Heart InstituteUniversity of North Carolina at Chapel HillChapel HillUSA
  2. 2.Department of Medicine, Division of Hematology and Oncology, McAllister Heart InstituteUniversity of North Carolina at Chapel HillChapel HillUSA

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