Molecular Medicine

, Volume 17, Issue 1–2, pp 36–40 | Cite as

Serum Leptin, Adiponectin and Tumor Necrosis Factor-α in Hyperlipidemic Rats with/without Concomitant Diabetes Mellitus

  • Angeliki Margoni
  • Despoina N. Perrea
  • Ioannis Vlachos
  • Georgia Prokopaki
  • Alkistis Pantopoulou
  • Lambros Fotis
  • Maria Kostaki
  • Athanasios G. Papavassiliou
Research Article


We compared the lipid profiles and serum levels of leptin, adiponectin and tumor necrosis factor-α (TNF-α) in rats with/without hyperlipidemia and with/without concomitant diabetes mellitus. Forty 10-wk-old male Wistar rats were divided into four groups. Groups A and C received standard food for 12 wks. Groups B and D received a high-fat diet enriched with 2% additional cholesterol. Moreover, insulin-deficient (type I) diabetes mellitus was induced in rats in groups C and D with intraperitoneal injections of streptozotocin. Fasting serum leptin levels were decreased in diabetic groups (groups C and D) compared with controls. Fasting serum adiponectin levels were decreased in groups C and D compared with group A. Serum TNF-α levels were augmented in groups B and D, those fed with an atherogenic diet. By contrast, TNF-α levels were decreased in group C. Our data suggest that serum leptin, adiponectin and TNF-α levels may serve as markers of obesity and type I diabetes mellitus.



This project was partially supported by the Special Account for Research Grants of the University of Athens (“Kapodistrias” Program). We thank I Habeos for helpful comments and suggestions.


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Copyright information

© The Feinstein Institute for Medical Research 2011

Authors and Affiliations

  • Angeliki Margoni
    • 1
  • Despoina N. Perrea
    • 2
  • Ioannis Vlachos
    • 2
  • Georgia Prokopaki
    • 2
  • Alkistis Pantopoulou
    • 2
  • Lambros Fotis
    • 2
  • Maria Kostaki
    • 1
  • Athanasios G. Papavassiliou
    • 1
  1. 1.Department of Biological Chemistry Medical SchoolUniversity of AthensAthensGreece
  2. 2.Laboratory for Experimental Surgery and Surgical Research “NS Christeas”University of Athens Medical SchoolAthensGreece

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