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Molecular Medicine

, Volume 17, Issue 1–2, pp 41–47 | Cite as

Identification of Phospholipid Scramblase 1 as a Biomarker and Determination of Its Prognostic Value for Colorectal Cancer

  • Yung-Bin Kuo
  • Chung-Chuan Chan
  • C. Allen Chang
  • Chung-Wei Fan
  • Ray-Ping Hung
  • Ya-Shu Hung
  • Kuei-Tien Chen
  • Jau-Song Yu
  • Yu-Sun Chang
  • Err-Cheng Chan
Research Article

Abstract

The purpose of this study was to examine the expression of phospholipid scramblase 1 (PLSCR1) in tumor tissues and plasma specimens of patients with colorectal cancer (CRC), as well as analyze its association with clinical parameters. The expression levels of PLSCR1 protein in 104 matched CRC and adjacent normal tissue sections and 50 pairs of CRC tissue blocks were determined by use of immunohistochemical and Western blot analyses, respectively. To evaluate the diagnostic potential of PLSCR1, the plasma levels of PLSCR1 were investigated in 111 additional subjects (59 CRC patients and 52 healthy controls) by Western blot. PLSCR1 was overexpressed in malignant adenocarcinoma tissues compared with normal colorectal mucosa (P < 0.001). In addition, the plasma level of PLSCR1 was not only significantly elevated in CRC patients compared with healthy individuals (P < 0.001), but it was also substantially increased in early stage CRC (P < 0.001). Importantly, the overall sensitivity and specificity of PLSCR1 for CRC detection were 80% and 59.6%, respectively. The area under the ROC curve of PLSCR1 for CRC diagnosis is 0.75, which increases to 0.8 if combined with the measurement of carcinoembryonic antigen. Univariate analysis with the Cox regression model revealed that elevated PLSCR1 expression indicated a poor prognosis for CRC. This study showed that PLSCR1 protein levels were significantly elevated in both the cancer tissue and plasma of CRC patients. Moreover, the plasma levels of PLSCR1 were significantly elevated in patients with early stage CRC compared with healthy individuals, suggesting that PLSCR1 might be used as a noninvasive serological diagnostic and prognostic biomarker for CRC.

Notes

Acknowledgments

This work was supported by grants (CMRPD180271 and CMRPG371431) from Chang Gung University and Chang Gung Memorial Hospital, Taiwan. CA Chang thanks the National Science Council of the Republic of China (Taiwan) for financial support (grant number NSC-98-2113-M-010-001-MY3).

Supplementary material

10020_2011_1701041_MOESM1_ESM.tif (94 kb)
Supplementary material, approximately 95 KB.
10020_2011_1701041_MOESM2_ESM.doc (74 kb)
Supplementary material, approximately 75 KB.

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Copyright information

© The Feinstein Institute for Medical Research 2011

Authors and Affiliations

  • Yung-Bin Kuo
    • 1
  • Chung-Chuan Chan
    • 2
  • C. Allen Chang
    • 1
    • 3
  • Chung-Wei Fan
    • 4
  • Ray-Ping Hung
    • 5
  • Ya-Shu Hung
    • 5
  • Kuei-Tien Chen
    • 5
  • Jau-Song Yu
    • 6
  • Yu-Sun Chang
    • 7
  • Err-Cheng Chan
    • 5
  1. 1.College of Biological Science and TechnologyNational Chiao Tung UniversityHsinchuTaiwan
  2. 2.Department of GastroenterologyHsinchu Cathay General HospitalHsinchuTaiwan
  3. 3.Department of Biomedical Imaging and Radiological SciencesNational Yang-Ming UniversityTaipeiTaiwan
  4. 4.Department of Colorectal SurgeryChang Gung Memorial HospitalKeelungTaiwan
  5. 5.Department of Medical Biotechnology and Laboratory ScienceChang Gung UniversityTaoyuanTaiwan
  6. 6.Cell and Molecular BiologyChang Gung UniversityTaoyuanTaiwan
  7. 7.Molecular Medicine Research CenterChang Gung UniversityTaoyuanTaiwan

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