Endogenous Erythropoietin as Part of the Cytokine Network in the Pathogenesis of Experimental Autoimmune Encephalomyelitis
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Erythropoietin (EPO) is of great interest as a therapy for many of the central nervous system (CNS) diseases and its administration is protective in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). Endogenous EPO is induced by hypoxic/ischemic injury, but little is known about its expression in other CNS diseases. We report here that EPO expression in the spinal cord is induced in mouse models of chronic or relapsing-remitting EAE, and is prominently localized to motoneurons. We found a parallel increase of hypoxia-inducible transcription factor (HIF)-1α, but not HIF-2α, at the mRNA level, suggesting a possible role of non-hypoxic factors in EPO induction. EPO mRNA in the spinal cord was co-expressed with interferon (IFN)-γ and tumor necrosis factor (TNF), and these cytokines inhibited EPO production in vitro in both neuronal and glialcells. Given the known inhibitory effect of EPO on neuroinflammation, our study indicates that EPO should be viewed as part of the inflammatory/anti-inflammatory network in MS.
The interest in erythropoietin (EPO) as a neuroprotective mediator has grown since it was found that systemically administered EPO crosses the blood brain barrier and is protective in several animal models of disease, including cerebral and spinal cord traumatic or ischemic injury and peripheral neuropathies (1,2). EPO administration reduced damage-induced neuroinflammation, a common component of all these diseases (3), suggesting that EPO anti-inflammatory action might be important in its pharmacological activity. This hypothesis also is supported by the fact that administration of EPO is effective in experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis (MS), and a model of inflammatory disease of the central nervous system (CNS) (1,4-6). The importance of this observation was stressed by a clinical trial with EPO in MS patients (7).
These findings raised the question of the expression of endogenous EPO in CNS diseases. EPO typically is induced via the hypoxia-inducible transcription factor (HIF)-1 and HIF-2, and its elevation mediates the increased production of erythrocytes in hypoxic conditions (8). While originally thought to be produced only by the kidney, many tissues now are recognized as expressing EPO (8,9).
Microarray studies in MS brain revealed upregulation of genes involved in neural protective mechanisms known to be induced upon ischemic preconditioning, including HIF-1a and vascular endothelial growth factor (VEGF) receptor 1 (14). In addition, expression of HIF-1a has been found in brain lesions of a subtype of MS cases (15), suggesting that a hypoxia-like tissue injury may play a pathogenic role in MS.
Therefore, we investigated the expression of EPO in the spinal cord of mice using two models of EAE: a chronic model induced in C57BL/6 mice by immunization against a peptide of myelin oligodendrocyte glycoprotein (MOG) (6), and a relapsing-remitting model induced in SJL mice by immunizing against myelin proteolipid protein (PLP) peptide (16). Since we found that EPO is co-induced along with interferon (IFN)-γ and tumor necrosis factor (TNF), proinflammatory cytokines with a pathogenic role in EAE, we investigated the effect of IFN-γ and TNF on EPO expression in neuronal and glial cells. The results indicate that EPO is induced during EAE and is negatively regulated by IFN-γ and TNF.
Materials and Methods
Procedures involving animals and their care were conducted in conformity with the institutional guidelines in compliance with national and international laws and policies (17, 18, 19). The protocols for the proposed investigation were reviewed and approved by the Animal Care and Use Committees (IACUC) of the Mario Negri Institute for Pharmacological Research.
Chronic EAE was induced in female C57BL/6 mice (6, 7, 8 wks; Harlan, Bresso, MI, Italy) by subcutaneous (s.c.) immunization with 200 µg of MOG peptide 35–55 (Multiple Peptide Systems, San Diego, CA, USA) per mouse in incomplete Freund’s adjuvant (Sigma, St Louis, MO, USA) supplemented with 8 mg/mL of M. tuberculosis (H37Ra; Difco Laboratories, Detroit, MI, USA). Mice received 500 ng of pertussis toxin (Sigma) intravenously (i.v.) at the time of immunization and 48 h later. Clinical score was recorded daily as described elsewhere (6).
Relapsing-remitting EAE was induced in female 8- to 12-wk-old SJL mice (Charles River, Calco, LC, Italy) by s.c. immunization with PLP peptide 139–151 (100 µg/mouse, in incomplete Freund’s adjuvant, Sigma, containing 2 mg/mL of heat-killed M. tuberculosis, Difco) as described (16).
Total RNA was extracted from spinal cords or cell cultures using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA), retrotranscribed according to standard procedures, and amplified by real-time reverse-transcription (RT)-PCR. All procedures were performed on the ABI PRISM 7300 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). For quantification, we used the comparative threshold cycle (ΔCt) method, according to the manufacturer’s guidelines. EPO mRNA was analyzed by the TaqMan method, with gene expression assays for EPO and GAPDH (housekeeping gene) commercially available from Applied Biosystems. Data were expressed as EPO mRNA units, with a cDNA from mouse kidney used as a calibrator (or rat kidney when EPO mRNA was measured in rat glial cells). IFN-γ and TNF mRNA levels were analyzed by the SYBR Green method, with primer sequences for TNF, IFN-γ, and GAPDH as reported (20). Data were expressed as fold induction versus control mice, using one of the controls as a calibrator.
Animals were sacrificed by intracardial perfusion (21). EPO localization was evaluated along the whole length of the spinal cord. Sections (40 um-thick) were washed in phosphate-buffered saline (PBS), incubated in 10% blocking serum in PBS at room temperature and exposed to polyclonal rabbit anti-EPO (1:100, H-162, Santa Cruz Biotechnology, Heidelberg, Germany) in 2% goat sera at 4° C. After an overnight incubation, the sections were incubated with biotinylated goat anti-rabbit antibodies (1:100, Vector Laboratories Inc., Burlingame, CA, USA) for 60 min, revealed with avidin-biotin using a peroxidase kit (Vectastain Elite, Vector), and the signal visualized with diaminobenzidine (DAB; Sigma Chemical, Deisenhofen, Germany) under a light microscope.
Free floating immunofluorescence experiments were carried out on 30 umthick lumbar spinal cord (L2-L3) sections. Coronal sectioning was preferred to better evaluate the localization of the different markers in specific areas of the grey matter. EPO was detected with polyclonal rabbit anti-EPO and biotinylated goat anti-rabbit antibodies as described above, and the immunofluorescence revealed with Alexa 488 goat anti-rabbit secondary antibody (1:1000, Molecular Probes, Eugene, OR, USA). Neurons were stained with 530–615 NeuroTrace Fluorescent Nissl reagent (1:5000, Molecular Probes). Astrocytes were identified by a mouse anti-GFAP monoclonal antibody (1:1000, Immunological Science, Rome, Italy) followed by an Alexa 546 goat antimouse secondary antibody (1:1000, Molecular Probes). Sections were observed with an Olympus Fluoview microscope BX61 with confocal system FV500. To confirm the specificity of the signal, we checked that incubating with either a primary or a secondary antibody alone gave no staining (not shown).
Human Kelly neuroblastoma cells were cultured and stimulated with the HIF-inducer deferoxamine mesylate (DFX, Sigma) as reported (22), with or without recombinant human IFN-γ (HyCult Biotechnology, Uden, The Netherlands) or recombinant human TNF-α (R&D Systems, Minneapolis, MN, USA). After 24 h of incubation, EPO was measured in the supernatant by Enzyme-Linked ImmunoSorbent Assay (ELISA) (Quantikine EPO, R&D Systems, sensitivity 0.6 mU/mL). All samples were assayed in duplicate. Results were expressed as mU/mL.
Primary cultures of glial cells were prepared from 1- to 2-d-old newborn rats (Sprague-Dawley, Charles River) as described (3). Cells were treated for 24 h with the HIF-inducer dimethyloxalylglycine (DMOG, Frontier Scientific Inc., Logan, UT, USA), with or without recombinant rat IFN-Y (R&D Systems) or recombinant rat TNF-α (R&D Systems), then total RNA was extracted and analyzed for EPO expression by real-time RT-PCR.
EPO Expression Is Increased in the Spinal Cord of Mice with EAE
In SJL mice, PLP139-155-induced EAE is characterized by a relapsing-remitting type of disease (16). In this model, EPO expression was increased in the spinal cord at the peak of the disease, and remained elevated in the remission phase (Figure 1B).
Immunohistochemical Localization of EPO in the Spinal Cord
When Nissl was used to identify neurons (Figure 2D, red), EPO staining (Figure 2E, green) co-localized with it (Figure 2F, merge), showing a cytoplasmic localization. On the other hand, colocalization experiments using GFAP to stain astrocytes (Figure 2G, red) and EPO (Figure 2H, green) confirmed the lack of expression of EPO by astrocytes (Figure 2I, merge).
HIF-1α Expression Is Induced in EAE
IFN-γ and TNF Are Co-Induced with EPO and Inhibit its Expression in Neuronal and Glial Cells in vitro
Although immunolocalization of EPO in EAE suggests it is produced mainly by neurons, others reported that glial cells also express EPO (10,11,25,26). We thus studied the effect of IFN-γ and TNF on EPO expression in rat primary glial cells, where EPO was induced with the HIF-stabilizer DMOG. In these experiments, EPO was measured by RT-PCR because its levels in the culture supernatants were undetectable by ELISA. As shown in Figure 5, panels C and D, both IFN-γ and TNF markedly and dosedependently inhibited EPO expression in glial cells.
While administration of EPO is protective in models of EAE (1,4, 5, 6) and possibly in MS patients (7), as well as in other CNS diseases (1,2), induction of endogenous EPO in the CNS has only been reported for hypoxic/ischemic pathologies, where EPO is induced as a result of the activation of HIF by hypoxia (10, 11, 12).
Our study shows that EPO is expressed in the CNS of mice with EAE, and immunohistochemistry indicated that it was associated mainly with neurons. The finding that neuronal cells are the main source of EPO expression in the spinal cord is in agreement with a previous study in the development of CNS (27).
In consideration of the anti-inflammatory action of exogenous EPO administration in EAE (6), the induction of endogenous EPO reported here suggests that EPO may be part of a protective response, along with anti-inflammatory cytokines such as interleukin-4 (IL-4), interleukin-10 (IL-10), and transforming growth factor (TGF)-β—whose expression correlates with recovery from EAE (28, 29, 30). Of note, considering the neuronal localization of EPO expression, others reported that neurons can express neurotrophic or protective cytokines: motoneurons can express brain-derived neurotrophic factor (bDNF) (31), and TGF-β is expressed by neurons during the remission phase of EAE (30).
The protective action of EPO in MS may be exerted also on other pathways than the inflammatory cytokine network. In fact, others have shown upregulation of VEGF, another target of HIF-1α, in the spinal cords of rats with EAE (32) and in MS active plaques from autopsy samples (14,32), and it was suggested that upregulated VEGF in MS might contribute to the increased permeability of the bloodbrain barrier (32). In this context, EPO was reported to counteract the increase in permeability induced by VEGF in an in vitro model of blood-brain barrier (33).
The mechanisms that upregulate EPO expression in EAE remain to be elucidated. The hypoxia-induced upregulation of EPO is modulated at the transcriptional level by HIF. The predominant mode of HIF induction by hypoxia is at the level of protein stability. In particular, the a subunits of HIF (HIF-1α and HIF-2α) are stabilized and accumulate upon hypoxia (34). In addition, several in vivo studies showed increased levels of HIF-1α mRNA (35,36). We found that EPO induction in EAE is paralleled by an elevation of HIF-1α, but not HIF-2α, mRNA. Of note, PhH, that induces EPO expression in the CNS secondary to anemia (23), had no effect on HIF-1α mRNA even if EPO induction was more marked than in EAE, confirming previous studies showing that it acts by increasing HIF at the protein level (24). Indeed PhH even decreased HIF-2α expression, possibly through a negative feedback mechanism.
Recently, possible mechanisms for HIF-1α mRNA induction by hypoxia have been characterized (37), and it also has been reported that non-hypoxic inflammatory mediators, including reactive oxygen species, may activate HIF-1α at the transcriptional level, possibly through NF-κB induction (38). In particular, inflammatory stimuli can induce HIF-1α mRNA in macrophages (39) and microglial cells (40), and these mechanisms might play a role in the increase of EPO expression in EAE. Of note, the effect of inflammatory stimuli on HIF transcriptional activity in macrophages is mediated by specific increase of HIF-1α, and not HIF-2α, mRNA (39), paralleling our finding in the inflamed spinal cord n EAE.
This study was supported in part by the Kenneth S Warren Institute, Ossining, NY, the Fondazione CARIPLO, Milan, Italy (to PG), the French Centre National de la Recherche Scientifique (CNRS) and the French Ministère de L’Enseignement Supérieur et de la Recherche (to EP and M Bernaudin).
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