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Molecular Medicine

, Volume 13, Issue 9–10, pp 480–487 | Cite as

Fibrin Gel-Immobilized VEGF and bFGF Efficiently Stimulate Angiogenesis in the AV Loop Model

  • Andreas Arkudas
  • Jimmy Tjiawi
  • Oliver Bleiziffer
  • Lucia Grabinger
  • Elias Polykandriotis
  • Justus P. Beier
  • Michael Stürzl
  • Raymund E. Horch
  • Ulrich Kneser
Research Article

Abstract

The modulation of angiogenic processes in matrices is of great interest in tissue engineering. We assessed the angiogenic effects of fibrin-immobilized VEGF and bFGF in an arteriovenous loop (AVL) model in 22 AVLs created between the femoral artery and vein in rats. The loops were placed in isolation chambers and were embedded in 500 µL fibrin gel (FG) (group A) or in 500 µL FG loaded with 0.1 ng/µL VEGF and 0.1 ng/µL bFGF (group B). After two and four weeks specimens were explanted and investigated using histological, morphometrical, and ultramorphological [scanning electron microscope (SEM) of vascular corrosion replicas] techniques. In both groups, the AVL induced formation of densely vascularized connective tissue with differentiated and functional vessels inside the fibrin matrix. VEGF and bFGF induced significantly higher absolute and relative vascular density and a faster resorption of the fibrin matrix. SEM analysis in both groups revealed characteristics of an immature vascular bed, with a higher vascular density in group B. VEGF and bFGF efficiently stimulated sprouting of blood vessels in the AVL model. The implantation of vascular carriers into given growth factor-loaded matrix volumes may eventually allow efficient generation of axially vascularized, tissue-engineered composites.

Notes

Acknowledgments

This study was supported by research grants from Xue-Hong and Hans Georg Geis, Baxter Healthcare Corporation, as well as the University of Erlangen (ELAN Program). The authors thank Professor Peter Greil and Mr. Peter Reinhard for production of the Teflon chambers. This work contains parts of Ms. Lucia Grabinger’s doctoral thesis. The authors thank Mr. Tobias Fey for preparation of the SEM specimens. The authors also thank Kristina Heidner and Simon Euler for their technical assistance.

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Copyright information

© Feinstein Institute for Medical Research 2007

Authors and Affiliations

  • Andreas Arkudas
    • 1
  • Jimmy Tjiawi
    • 1
  • Oliver Bleiziffer
    • 1
  • Lucia Grabinger
    • 1
  • Elias Polykandriotis
    • 1
  • Justus P. Beier
    • 1
  • Michael Stürzl
    • 2
  • Raymund E. Horch
    • 1
  • Ulrich Kneser
    • 1
  1. 1.Department of Plastic and Hand SurgeryUniversity of Erlangen Medical CenterErlangenGermany
  2. 2.Department of Molecular and Experimental SurgeryUniversity of Erlangen Medical CenterErlangenGermany

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