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Molecular Medicine

, Volume 13, Issue 7–8, pp 415–421 | Cite as

Nicotinamide N-Methyltransferase Upregulation Inversely Correlates with Lymph Node Metastasis in Oral Squamous Cell Carcinoma

  • Davide Sartini
  • Andrea Santarelli
  • Valentina Rossi
  • Gaia Goteri
  • Corrado Rubini
  • Domenico Ciavarella
  • Lorenzo Lo Muzio
  • Monica Emanuelli
Proceedings

Abstract

We investigated expression levels of Nicotinamide N-Methyltransferase (NNMT), an enzyme Involved In the biotransformation of many drugs and xenobiotic compounds, in oral squamous cell carcinoma (OSCC). Measurements were performed by semiquantitative RT-PCR and quantitative real-time PCR in tumor and matched adjacent healthy tissue. Interestingly, NNMT was upregulated in most of the favorable OSCCs, while no marked NNMT expression alterations between tumor and normal mucosa were detected in most of the unfavorable OSCCs. Western blot and immunohistochemical analyses also were performed and the relationship between tumor characteristics and NNMT levels in OSCC were studied to evaluate the effectiveness of NNMT as a prognostic marker in the squamous cell carcinoma of the oral cavity. In summary, the present study suggests that NNMT may have potential as a biomarker and a therapeutic target for OSCC.

Notes

Acknowledgments

We gratefully acknowledge Dr Richard Weinshilboum, Mayo Clinic, Rochester, MN, for providing NNMT antibody.

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Copyright information

© Feinstein Institute for Medical Research 2007

Authors and Affiliations

  • Davide Sartini
    • 1
  • Andrea Santarelli
    • 2
  • Valentina Rossi
    • 1
  • Gaia Goteri
    • 3
  • Corrado Rubini
    • 3
  • Domenico Ciavarella
    • 4
  • Lorenzo Lo Muzio
    • 4
  • Monica Emanuelli
    • 1
  1. 1.Istituto di Biotecnologie BiochimicheUniversità Politecnica delle MarcheAnconaItaly
  2. 2.Institute of Dentistry and Stomatological Sciences, Section of Pathological Anatomy and HistopathologyPolytechnic University of MarcheAnconaItaly
  3. 3.Department of Neurosciences, Section of Pathological Anatomy and HistopathologyPolytechnic University of MarcheAnconaItaly
  4. 4.Department of Surgical SciencesUniversity of FoggiaFoggiaItaly

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