Molecular Medicine

, Volume 13, Issue 1–2, pp 14–21 | Cite as

Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell

  • Zhi Yao
  • Xu-chun Che
  • Rong Lu
  • Min-na Zheng
  • Zhi-feng Zhu
  • Jin-ping Li
  • Xu Jian
  • Lin-xi Shi
  • Jun-yan Liu
  • Wen-yuan Gao
Research Article


Tyroserleutide (YSL) is an active, low-molecular-weight polypeptide, comprised of three amino acids, that has shown antitumor effects on human hepatocarcinoma BEL-7402 in vitro and in vivo. In this study, we evaluated the inhibition of YSL on invasion and adhesion of the mouse B16-F10 melanoma cell line by injecting B16-F10 cells into the tail veins of C57BL/6 mice to establish an experimental lung metastasis model. YSL inhibited B16-F10 cell metastasis to lung, reducing the number and area of metastasis lesions. When we treated B16-F10 cells with YSL (0.01, 0.1, 1, 10, or 100 µg/mL) in vitro, we found that YSL inhibited the proliferation of B16-F10 cells with a 28.11% rate of inhibition. YSL significantly decreased the adhesiveness of B16-F10 cells to Matrigel with a 29.15% inhibition rate; YSL also significantly inhibited the invasion of B16-F10 cells, producing an inhibition of 35.31%. By analyses with Western blot and real-time RT-PCR, we found that YSL markedly inhibited the expression of ICAM-1 in B16-F10 cells. These data suggest that YSL inhibits the growth, invasion, and adhesion of B16-F10 cells.



This study was supported by grants for the National High Technology Research and Development Program of China (2004AA2Z3170, 2005AA2Z3D40) (863 Program), the National Basic Research Program (2003CCA04300) (973 Program) and a project grant (03007) from the Department of Education of China.


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Copyright information

© Feinstein Institute for Medical Research 2007

Authors and Affiliations

  • Zhi Yao
    • 1
    • 2
  • Xu-chun Che
    • 2
  • Rong Lu
    • 2
    • 3
  • Min-na Zheng
    • 2
  • Zhi-feng Zhu
    • 3
  • Jin-ping Li
    • 3
  • Xu Jian
    • 2
  • Lin-xi Shi
    • 2
  • Jun-yan Liu
    • 2
  • Wen-yuan Gao
    • 1
  1. 1.The College of Pharmaceuticals and BiotechnologyTianjin UniversityTianjinChina
  2. 2.Department of ImmunologyTianjin Medical UniversityTianjinChina
  3. 3.Shenzhen Kangzhe Pharmaceutical Co., Ltd.Shenzhen, GuangdongChina

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