Advertisement

Molecular Medicine

, Volume 13, Issue 1–2, pp 14–21 | Cite as

Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell

  • Zhi Yao
  • Xu-chun Che
  • Rong Lu
  • Min-na Zheng
  • Zhi-feng Zhu
  • Jin-ping Li
  • Xu Jian
  • Lin-xi Shi
  • Jun-yan Liu
  • Wen-yuan Gao
Research Article

Abstract

Tyroserleutide (YSL) is an active, low-molecular-weight polypeptide, comprised of three amino acids, that has shown antitumor effects on human hepatocarcinoma BEL-7402 in vitro and in vivo. In this study, we evaluated the inhibition of YSL on invasion and adhesion of the mouse B16-F10 melanoma cell line by injecting B16-F10 cells into the tail veins of C57BL/6 mice to establish an experimental lung metastasis model. YSL inhibited B16-F10 cell metastasis to lung, reducing the number and area of metastasis lesions. When we treated B16-F10 cells with YSL (0.01, 0.1, 1, 10, or 100 µg/mL) in vitro, we found that YSL inhibited the proliferation of B16-F10 cells with a 28.11% rate of inhibition. YSL significantly decreased the adhesiveness of B16-F10 cells to Matrigel with a 29.15% inhibition rate; YSL also significantly inhibited the invasion of B16-F10 cells, producing an inhibition of 35.31%. By analyses with Western blot and real-time RT-PCR, we found that YSL markedly inhibited the expression of ICAM-1 in B16-F10 cells. These data suggest that YSL inhibits the growth, invasion, and adhesion of B16-F10 cells.

Notes

Acknowledgments

This study was supported by grants for the National High Technology Research and Development Program of China (2004AA2Z3170, 2005AA2Z3D40) (863 Program), the National Basic Research Program (2003CCA04300) (973 Program) and a project grant (03007) from the Department of Education of China.

References

  1. 1.
    Pan Y, Song QL, Lin YH, Lu N, Yu HM, Li XJ. (2005) GLB prevents tumor metastasis of Lewis lung carcinoma by inhibiting tumor adhesion actions. Acta Pharmacol. Sin. 26:881–6.CrossRefGoogle Scholar
  2. 2.
    Muehlemann M, Miller KD, Dauphinee M, Mizejewski GJ. (2005) Review of growth inhibitory peptide as a biotherapeutic agent for tumor growth, adhesion, and metastasis. Cancer Metastasis Rev. 24:441–67.CrossRefGoogle Scholar
  3. 3.
    Yamamoto Y, Tsutsumi Y, Mayumi T. (2002) Molecular design of bioconjugated cell adhesion pep-tide with a water-soluble polymeric modifier for enhancement of antimetastatic effect. Curr. Drug Targets 3:123–30.CrossRefGoogle Scholar
  4. 4.
    Kato R et al. (2002) A new type of antimetastatic peptide derived from fibronectin. Clin. Cancer Res. 8:2455–62.PubMedGoogle Scholar
  5. 5.
    Lu R et al. (2005) Experimental study of the inhibition of human hepatocarcinoma Bel7402 cells by the tripeptide tyroserleutide (YSL). Cancer Chemother. Pharmacol. 57:1–9.Google Scholar
  6. 6.
    Kawasaki M, Maeda T, Hanasawa K, Ohkubo I, Tani T. (2003) Effect of His-Gly-Lys motif derived from domain 5 of high molecular weight kininogen on suppression of cancer metastasis both in vitro and in vivo. J. Biol. Chem. 278:49301–7.CrossRefGoogle Scholar
  7. 7.
    Okegawa T, Pong RC, Li Y, Hsieh JT. (2004) The role of cell adhesion molecule in cancer progression and its application in cancer therapy Acta Biochim. Pol. 445–57.Google Scholar
  8. 8.
    Mummert ME, Mummert DI, Ellinger L, Takashima A. (2003) Functional roles of hyaluronan in B16-F10 melanoma growth and experimental metastasis in mice. Mol. Cancer Ther. 2:295–300.PubMedGoogle Scholar
  9. 9.
    McGary EC et al. (2003) Afully human antimelanoma cellular adhesion molecule/MUC18 antibody inhibits spontaneous pulmonary metastasis of osteosarcoma cells in vivo. Clin. Cancer Res. 9:6560–6.PubMedGoogle Scholar
  10. 10.
    Sullivan RM, Stone M, Marshall JF, Uberall F, Rotenberg SA. (2000) Photo-induced inactivation of protein kinase calpha by dequalinium inhibits motility of murine melanoma cells. Mol. Pharmacol. 58:729–37.CrossRefGoogle Scholar
  11. 11.
    Zhang XM, Huang SP, Xu Q. (2004) Quercetin inhibits the invasion of murine melanoma B16-BL6 cells by decreasing pro-MMP-9 via the PKC pathway. Cancer Chemother. Pharmacol. 53:82–8.CrossRefGoogle Scholar
  12. 12.
    Dussault AA, Pouliot M. (2006) Rapid and simple comparison of messenger RNA levels using real-time PCR. Biol. Proc. Online 8:1–10.CrossRefGoogle Scholar
  13. 13.
    Livak KJ, Schmittgen TD. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-Δ Δ CT method. Methods 25:402–8.CrossRefGoogle Scholar
  14. 14.
    Usui T, Ishida S, Yamashiro K, et al. (2004) VEGF164 (165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2. Invest. Ophthalmol. Vis. Sci. 45:368–74.CrossRefGoogle Scholar
  15. 15.
    Yamaguchi H et al. (2003) Sphingosine-1-phosphate receptor subtype-specific positive and negative regulation of Rac and haematogenous metastasis of melanoma cells. Biochem. J. 374:715–22.CrossRefGoogle Scholar
  16. 16.
    Theiszova M, Jantova S, Dragunova J, Grznarova P, Palou M. (2005) Comparison the cytotoxicity of hydroxyapatite measured by direct cell counting and MTT test in murine fibroblast NIH-3T3 cells. Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub. 149:393–6.CrossRefGoogle Scholar
  17. 17.
    Zheng HQ, Liu DY. (2003) [Anti-invasive and anti-metastatic effect of ampelopsin on melanoma]. Ai Zheng 22:363–7.PubMedGoogle Scholar
  18. 18.
    Ogasawara M, Matsunaga T, Takahashi S, Saiki I, Suzuki H. (2002) Anti-invasive and metastatic activities of evodiamine. Biol. Pharm. Bull. 25:1491–3.CrossRefGoogle Scholar
  19. 19.
    Tachimori A et al. (2005) Up regulation of ICAM-1 gene expression inhibits tumor growth and liver metastasis in colorectal carcinoma. Eur. J. Cancer 41:1802–10.CrossRefGoogle Scholar
  20. 20.
    Yashiro M, Sunami T, Hirakawa K. (2005) CD54 expression is predictive for lymphatic spread in human gastric carcinoma. Dig. Dis. Sci. 50:2224–30.CrossRefGoogle Scholar
  21. 21.
    Lu H et al. (2005) Angiogenesis inhibitor Z24 induces endothelial cell apoptosis and suppresses tumor growth and metastasis. J. Pharmacol. Sci. 97:533–40.CrossRefGoogle Scholar
  22. 22.
    Ren B, Hoti N, Rabasseda X, Wang YZ, Wu M. (2003) The antiangiogenic and therapeutic implications of endostatin. Methods Find Exp. Clin. Pharmacol. 25:215–24.CrossRefGoogle Scholar
  23. 23.
    Liotta LA et al. (1988) Biochemical mechanisms of tumor invasion and metastases. Prog. Clin. Biol. Res. 256:3–16.PubMedGoogle Scholar
  24. 24.
    Wang Y et al. (2004) Inhibitors of 5-lipoxygenase inhibit expression of intercellular adhesion molecule-1 in human melanoma cells. Acta Pharmacol. Sin. 25:672–7.PubMedGoogle Scholar
  25. 25.
    Stratz T, Kees F, Muller W. (2003) Do cytochrome enzymes influence the therapeutic effect of tropisetron in fibromyalgia? Drugs Exp. Clin. Res. 29:91–4.PubMedGoogle Scholar
  26. 26.
    Patya M et al. (2004) Allicin stimulates lymphocytes and elicits an antitumor effect: a possible role of p21ras. Int. Immunol. 16:275–81.CrossRefGoogle Scholar
  27. 27.
    Curnis F, Gasparri A, Sacchi A, Cattaneo A, Magni F, Corti A. (2005) Targeted delivery of IFNgamma to tumor vessels uncouples antitumor from counterregulatory mechanisms. Cancer Res. 65:2906–13.CrossRefGoogle Scholar
  28. 28.
    Barnard R, Gurevich KG. (2005) In vitro bioassay as a predictor of in vivo response. Theor. Biol. Med. Model 2:3.CrossRefGoogle Scholar
  29. 29.
    Paulsen JE. (1993) The synthetic colon peptide pyroGlu-His-GlyOH inhibits growth of human colon carcinoma cells (HT-29) transplanted subcutaneously into athymic mice. Carcinogenesis 14:1719–21.CrossRefGoogle Scholar
  30. 30.
    Setyono-Han B, Henkelman MS, Foekens JA, Klijn GM. (1987) Direct inhibitory effects of somatostatin (analogues) on the growth of human breast cancer cells. Cancer Res. 47:1566–70.PubMedGoogle Scholar

Copyright information

© Feinstein Institute for Medical Research 2007

Authors and Affiliations

  • Zhi Yao
    • 1
    • 2
  • Xu-chun Che
    • 2
  • Rong Lu
    • 2
    • 3
  • Min-na Zheng
    • 2
  • Zhi-feng Zhu
    • 3
  • Jin-ping Li
    • 3
  • Xu Jian
    • 2
  • Lin-xi Shi
    • 2
  • Jun-yan Liu
    • 2
  • Wen-yuan Gao
    • 1
  1. 1.The College of Pharmaceuticals and BiotechnologyTianjin UniversityTianjinChina
  2. 2.Department of ImmunologyTianjin Medical UniversityTianjinChina
  3. 3.Shenzhen Kangzhe Pharmaceutical Co., Ltd.Shenzhen, GuangdongChina

Personalised recommendations