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European Journal of Dermatology

, Volume 27, Issue 6, pp 590–598 | Cite as

Immunoreactivity to CYP24A1, but not vitamin D receptor, is increased in mast cells of keratinocyte skin cancers

  • Antti Kaukinen
  • Hanna Siiskonen
  • Jukka Pelkonen
  • Ilkka T. HarvimaEmail author
Investigative report
  • 26 Downloads

Abstract

Background

In mouse skin models, mast cells have been shown to express vitamin D receptor (VDR) that can mediate the immunosuppressive effects of ultravioletBradiation and vitamin D3. However,VDR activation leads to the expression of CYP24A1, a hydroxylase that can inactivate vitamin D3 metabolites.

Objectives

To examine immunoreactivity to VDR and CYP24A1 in mast cells from normal human skin, keratinocyte skin cancers, and disorders of chronic inflammation.

Materials & methods

Frozen biopsies were collected from the non-lesional and lesional skin of patients with actinic keratosis (AK), Bowen’s disease/ squamous cell carcinoma (SCC), basal cell carcinoma (BCC), and psoriasis. The expression of VDR and CYP24A1 in tryptase-positive mast cells was analysed using double-staining methods.

Results

Less than 0.5% of the mast cells were immunoreactive to VDR in both the non-lesional and lesional skin for all disease groups. In non-lesional skin, only 0.5-2.9% of the mast cells were immunopositive for CYP24A1, however, the percentage of mast cells containing CYP24A1 was significantly increased in lesional skin of AK, SCC, and BCC. In contrast to human skin, LAD2 mast cells cultured from a patient with mast cell sarcoma/leukaemia revealed that about 34% and 6.5% of the cells were immunopositive forVDRand CYP24A1, respectively.

Conclusion

Whereas a very small proportion of mast cells in human skin express VDR and CYP24A1, the proportion of mast cells expressing CYP24A1 in keratinocyte skin cancers is increased; the mechanism underlying this is unclear.

Key words

mast cell vitamin D skin cancer psoriasis 

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Copyright information

© JLE/Springer 2018

Authors and Affiliations

  • Antti Kaukinen
    • 1
  • Hanna Siiskonen
    • 1
  • Jukka Pelkonen
    • 2
  • Ilkka T. Harvima
    • 1
    Email author
  1. 1.Department of DermatologyUniversity of Eastern Finland and Kuopio University HospitalKuopioFinland
  2. 2.Department of Clinical MicrobiologyUniversity of Eastern FinlandKuopioFinland

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