Advertisement

Journal of Zhejiang University-SCIENCE B

, Volume 20, Issue 7, pp 613–616 | Cite as

Hypolipidemic activity of low-cholesterol ovum oil of Rana chensinensis and phytosterol (stigmasterol) in rats

  • Chang-hui Zhao
  • Chao Zhao
  • Hai-qing Ye
  • Chun-yu Xi
  • Zheng-ping Zou
  • Xin-hui Zhou
  • Ping-ping Hou
  • Tie-hua ZhangEmail author
Correspondence
  • 20 Downloads

低胆固醇林蛙卵油和植物甾醇(豆甾醇)在大鼠中的降脂作用

中文概要

目的:探索林蛙副产物林蛙卵油的开发价值。

创新点:首次验证了低胆固醇林蛙卵油的减重降脂功能。

方法:Sprague-Dawley 大鼠连续被灌胃8 周后,分成8组:(1)对照组喂食标准饲料;(2)高脂组喂食60%高脂饲料;(3)阳性对照组喂食3.33 mg/(kg·d)辛伐他汀;(4)OORC 组喂食2 g/(kg·d)的低胆固醇林蛙卵油(OORC);(5)植物甾醇组喂食0.48 g/(kg·d) 的豆甾醇;( 6 ) 低剂量组喂食1 g/(kg·d)的低胆固醇OORC 和0.24 g/(kg·d)的豆甾醇;(7)中剂量组喂食2 g/(kg·d)的低胆固醇OORC 和0.48 g/(kg·d)的豆甾醇;(8)高剂量组喂食4 g/(kg·d)的低胆固醇OORC 和0.96 g/(kg·d)的豆甾醇。经过不同方法处理后,比较各组大鼠的体重、器官指数,以及血清中总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白浓度。同时,对动物肝脏进行组织切片染色并评价损伤情况。

结论:低胆固醇林蛙卵油或者其与植物甾醇(如豆甾醇)联用可以预防高脂饮食诱导的增重、肝损伤和高血脂症状。

关键词

林蛙卵油 林蛙 肥胖 高血脂 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Supplementary material

11585_2019_16_MOESM1_ESM.pdf (525 kb)
Hypolipidemic activity of low-cholesterol ovum oil of Rana chensinensis and phytosterol (stigmasterol) in rats
11585_2019_16_MOESM2_ESM.pdf (122 kb)
Hypolipidemic activity of low-cholesterol ovum oil of Rana chensinensis and phytosterol (stigmasterol) in rats

References

  1. Bahmani M, Mirhoseini M, Shirzad H, et al., 2015. A review on promising natural agents effective on hyperlipidemia. J Evid Based Complementary Altern Med, 20(3):228–238.  https://doi.org/10.1177/2156587214568457 CrossRefGoogle Scholar
  2. Bao Y, Jiang W, Liu D, et al., 2016. Advances in pharmacological research of Chinese forest frog. Mod Chin Med, 18(8):1077–1080 (in Chinese).  https://doi.org/10.13313/j.issn.1673-4890.2016.8.031 Google Scholar
  3. Chen JT, Wesley R, Shamburek RD, et al., 2005. Meta-analysis of natural therapies for hyperlipidemia: plant sterols and stanols versus policosanol. Pharmacotherapy, 25(2):171–183.  https://doi.org/10.1592/phco.25.2.171.56942 CrossRefGoogle Scholar
  4. Cholesterol Treatment Trialists’ (CTT) Collaboration, 2015. Efficacy and safety of LDL-lowering therapy among men and women: meta-analysis of individual data from 174 000 participants in 27 randomised trials. Lancet, 385(9976):1397–1405.  https://doi.org/10.1016/S0140-6736(14)61368-4 CrossRefGoogle Scholar
  5. Ji XF, Lin F, Qiu FP, 2013. Optimization on aqueous enzymatic extraction conditions of forest frog egg protein by response surface method. J Changchun Univ Technol (Nat Sci Ed), 34(2):137–144 (in Chinese).  https://doi.org/10.3969/j.issn.1674-1374.2013.02.005 Google Scholar
  6. Jin LL, Wang QY, Lou H, et al., 2004. The analysis and research of components of fatty acid in the ovum oil of Rana chensinensis. Food Sci, 25(10):232–234 (in Chinese).  https://doi.org/10.3321/j.issn:1002-6630.2004.10.055 Google Scholar
  7. Nelson RH, 2013. Hyperlipidemia as a risk factor for cardiovascular disease. Prim Care, 40(1):195–211.  https://doi.org/10.1016/j.pop.2012.11.003 CrossRefGoogle Scholar
  8. Park Y, Carr TP, 2013. Unsaturated fatty acids and phytosterols regulate cholesterol transporter genes in Caco-2 and HepG2 cell lines. Nutrit Res, 33(2):154–161.  https://doi.org/10.1016/j.nutres.2012.11.014 CrossRefGoogle Scholar
  9. Wang ZY, Zhao YY, Su TT, et al., 2015. Characterization and antioxidant activity in vitro and in vivo of polysaccharide purified from Rana chensinensis skin. Carbohydr Polym, 126:17–22.  https://doi.org/10.1016/j.carbpol.2015.03.031 CrossRefGoogle Scholar
  10. Yu L, Morishima C, Ioannou GN, 2013. Dietary cholesterol intake is associated with progression of liver disease in patients with chronic hepatitis C: analysis of the hepatitis C antiviral long-term treatment against cirrhosis trial. Clin Gastroenterol Hepatol, 11(12):1661–1666.  https://doi.org/10.1016/j.cgh.2013.05.018 CrossRefGoogle Scholar
  11. Zhang T, Zhao C, Zhao C, et al., 2019. Optimization of conditions for cholesterol removal from Rana chensinensis ovum oil. Curr Topics Nutraceut Res, 17(2):135–139.Google Scholar

Copyright information

© Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Chang-hui Zhao
    • 1
  • Chao Zhao
    • 1
  • Hai-qing Ye
    • 1
  • Chun-yu Xi
    • 1
  • Zheng-ping Zou
    • 1
  • Xin-hui Zhou
    • 1
  • Ping-ping Hou
    • 1
  • Tie-hua Zhang
    • 1
    Email author
  1. 1.College of Food Science and EngineeringJilin UniversityChangchunChina

Personalised recommendations