Abstract
Helicobacter pylori infection is related to the development of gastric diseases. Our previous studies showed that high thioredoxin-1 (Trx1) expression in H. pylori can promote gastric carcinogenesis. To explore the underlying molecular mechanisms, we performed an isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis of stomach tissues from Mongolian gerbil infected with H. pylori expressing high and low Trx1. Differences in the profiles of the expressed proteins were analyzed by bioinformatics and verified using Western blot analysis. We found three candidate proteins, 14-3-3α/β, glutathione-S-transferase (GST), and heat shock protein 70 (HSP70), in high Trx1 tissues compared with low Trx1 tissues and concluded that cellular stress and redox activityrelated proteins were involved in the pathogenesis of gastric cancer associated with H. pylori Trx1.
中文概要
目的
探索高表达硫氧还蛋白-1(Trx1)的幽门螺杆菌在致胃黏膜癌变过程中的作用机制。
创新点
首次分析临床分离菌株感染蒙古沙土鼠的胃黏膜疾病动物模型,首次对不同菌株感染所致的胃黏膜癌变相关分子机制进行探索。
方法
用同位素标记相对和绝对定量(iTRAQ)蛋白质组学及生物信息学分析方法进行分析,并用免疫印迹法(Western blot)对重要分子进行验证。
结论
高表达Trx1 的幽门螺杆菌在致胃黏膜癌变过程中,三个重要的细胞应激和氧化还原活性蛋白包括14-3-3α/β、谷胱甘肽转移酶(GST)和热休克蛋白(HSP70)参与致病。
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Acknowledgements
The authors thank Kai XIONG of Peking University (Beijing, China) for providing technological help.
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Project supported by the Key Laboratory for Helicobacter pylori Infection and Upper Gastrointestinal Diseases, Beijing Key Laboratory (No. BZ0371) and the National Natural Science Foundation of China (Nos. 81700496, 81270475, and 30770980)
Electronic supplementary materials: The online version of this article (https://doi.org/10.1631/jzus.B1700456 ) contains supplementary materials, which are available to authorized user
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Cellular stress and redox activity proteins are involved in gastric carcinogenesis associated with Helicobacter pylori infection expressing high levels of thioredoxin-1
11585_2018_456_MOESM2_ESM.xls
Cellular stress and redox activity proteins are involved in gastric carcinogenesis associated with Helicobacter pylori infection expressing high levels of thioredoxin-1
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Cellular stress and redox activity proteins are involved in gastric carcinogenesis associated with Helicobacter pylori infection expressing high levels of thioredoxin-1
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Cellular stress and redox activity proteins are involved in gastric carcinogenesis associated with Helicobacter pylori infection expressing high levels of thioredoxin-1
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Shi, Yy., Zhang, J., Zhang, T. et al. Cellular stress and redox activity proteins are involved in gastric carcinogenesis associated with Helicobacter pylori infection expressing high levels of thioredoxin-1. J. Zhejiang Univ. Sci. B 19, 750–763 (2018). https://doi.org/10.1631/jzus.B1700456
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DOI: https://doi.org/10.1631/jzus.B1700456
Key words
- Thioredoxin-1 (Trx1)
- Helicobacter pylori
- Gastric carcinogenesis
- Proteomics
- Isobaric tags for relative and absolute quantitation (iTRAQ)