Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences
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For a long time, classification of Demodex mites has been based mainly on their hosts and phenotypic characteristics. A new subspecies of Demodex folliculorum has been proposed, but not confirmed. Here, cox1 partial sequences of nine isolates of three Demodex species from two geographical sources (China and Spain) were studied to conduct molecular identification of D. folliculorum. Sequencing showed that the mitochondrial cox1 fragments of five D. folliculorum isolates from the facial skin of Chinese individuals were 429 bp long and that their sequence identity was 97.4%. The average sequence divergence was 1.24% among the five Chinese isolates, 0.94% between the two geographical isolate groups (China (5) and Spain (1)), and 2.15% between the two facial tissue sources (facial skin (6) and eyelids (1)). The genetic distance and rate of third-position nucleotide transition/transversion were 0.0125, 2.7 (3/1) among the five Chinese isolates, 0.0094, 3.1 (3/1) between the two geographical isolate groups, and 0.0217, 4.4 (3/1) between the two facial tissue sources. Phylogenetic trees showed that D. folliculorum from the two geographical isolate groups did not form sister clades, while those from different facial tissue sources did. According to the molecular characteristics, it appears that subspecies differentiation might not have occurred and that D. folliculorum isolates from the two geographical sources are of the same population. However, population differentiation might be occurring between isolates from facial skin and eyelids.
Key wordsDemodex folliculorum cox1 partial sequences Divergence Genetic relationship Phylogenetic tree
CLC numberR384.4; R34
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- Li, C.P., 2009. Medical Arthropodology. People’s Medical Publishing House, Beijing, China (in Chinese).Google Scholar
- Liu, S.F., Chen, L.L., Dai, F.Q., Zhuang, Z.M., 2010. Application of DNA barcoding gene cox1 for classifying family Sciagenidae. Oceanol. Limnol. Sin., 41(2):223–231 (in Chinese).Google Scholar
- Toops, E., Blagburn, B., Lenaghan, S., Kennis, R., MacDonald, J., Dykstra, C., 2010. Extraction and characterization of DNA from Demodex canis. J. Appl. Res. Vet. Med., 8(1):31–43.Google Scholar
- Tsao, W.C., Yeh, W.B., 2008. DNA-based discrimination of subspecies of swallowtail butterflies (Lepidoptera: Papilioninae) from Taiwan. Zool. Stud., 47(5):633–643.Google Scholar
- Wang, Y.P., Li, P., Bing, G.Q., 1998. A case report of human dermatitis caused by Canine Demodex. J. N. Bethune Univ. Med. Sci., 24(3):265 (in Chinese).Google Scholar
- Xie, H.X., Liu, S.L., Xu, Y.H., Xu, M.Q., 1982. Taxonomy of the family Demodicidae and new subspecies. Acta Zootaxon. Sin., 7(3):265–269 (in Chinese).Google Scholar
- Zhao, Y.E., Cheng, H., 2009. RAPD analysis and sequence alignment of genomic DNA of hair follicle mites Demodex folliculorum and D. brevis (Acari: Demodicidae). Acta Entomol. Sin., 52(11):1273–1279 (in Chinese).Google Scholar
- Zhao, Y.E., Cheng, H., Xun, M., Wu, L.P., 2009b. Extraction and random primer PCR detection of genomic DNA of parasitic mites Demodex folliculorum and Demodex brevis (Acari: Demodicidae). Acta Entomol. Sin., 52(8): 929–933 (in Chinese).Google Scholar
- Zhao, Y.E., Wu, L.P., Hu, L., Xu, Y., Wang, Z.H., Liu, W.Y., 2012c. Sequencing for complete rDNA sequences (18S, ITS1, 5.8S, ITS2, and 28S rDNA) of Demodex and phylogenetic analysis of Acari based on 18S and 28S rDNA. Parasitol. Res., 111(5):2109–2114. [doi:10.1007/s00436-012-3058-8]PubMedCrossRefGoogle Scholar
- Zhou, J.Y., Zhang, Q., Tang, Y.L., Yu, F.Y., Zhao, S., 2010. On phylogenetic relationships of Teraponidae in coastal waters of China. Mar. Fish, 32(4):351–355 (in Chinese).Google Scholar