Abstract
We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat (STR) loci and the amelogenin locus, which are not included in the combined DNA index system (CODIS), in a Russian ethnic minority group from the Inner Mongolia Autonomous Region, China. A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction (PCR) system. Using capillary electrophoresis, the PCR products of the 21 STR loci were separated and genotyped. A total of 161 alleles were observed in the Russian ethnic minority group, and corresponding allelic frequencies ranged from 0.0044 to 0.5965. The 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population’s genetic background, for individual identification, and for paternity testing in forensic practice.
References
Bär, W., Brinkmann, B., Budowle, B., Carracedo, A., Gill, P., Lincoln, P., Mayr, W., Olaisen, B., 1997. DNA recommendations. Further report of the DNA commission of the ISFH regarding the use of short tandem repeat systems. Int. J. Legal Med., 110(4):175–176. [doi:10.1007/s004140050061]
El Ossmani, H., Bouchrif, B., Talbi, J., El Amri, H., Chafik, A., 2007. Gene diversity of 15 STR among the Arab speaking population of Rabat-Salé-Zemmour-Zaer. Antropo, 15:55–62 (in French).
El Ossmani, H., Talbi, J., Bouchrif, B., Chafik, A., 2009. Allele frequencies of 15 autosomal STR loci in the southern Morocco population with phylogenetic structure among worldwide populations. Legal Med., 11(3): 155–158. [doi:10.1016/j.legalmed.2009.01.053]
Guo, J.Z., Lu, Z.Y., Yu, L.J., Liu, F., Zhang, Q.X., Jiao, Z.P., Liu, Y.C., 2010. Polymorphism analysis and evaluation of 21 non CODIS STR loci in the Beijing Han population. Forensic Sci. Technol., 6:13–16 (in Chinese).
Hammond, H.A., Jin, L., Zhong, Y., Caskey, C.T., Chakraborty, R., 1994. Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am. J. Hum. Genet., 55(1):175–189.
Lu, S.H., Zheng, L.B., So, L.Y., Zhao, Z., 2005. A study of the physical characteristics of the Russ nationality. Acta Anthropol. Sin., 24(4):291–300.
Lu, S.H., Zheng, L.B., So, L.Y., Zhao, Z., 2006. A comparison of physical characteristics of Chinese Russ with that of five groups. J. Inn. Mong. Norm. Univ. Nat. Sci., 35(1): 99–102 (in Chinese).
Shen, C.M., Wang, H.D., Liu, W.J., Fan, S.L., Yang, G., Qin, H.X., Xie, T., Li, S.B., Yan, J.W., Zhu, B.F., 2012. Allelic diversity distributions of 21 autosomal short tandem repeat loci in a Chinese Bai ethnic group. Legal Med., 15(2): 109–113. [doi:10.1016/j.legalmed.2012.08.012]
Teng, Y., Zhang, F.X., Shen, C.M., Wang, H.D., Yan, J.W., Liu, J.L., 2012. Genetic variation of new 21 autosomal short tandem repeat loci in a Chinese Salar ethnic group. Mol. Biol. Rep., 39(2):1465–1470. [doi:10.1007/s11033-011-0883-2]
Tereba, A., 1999. Tools for Analysis of Population Statistics: Profiles in DNA, Vol 3. Available from http://www.promega.com/geneticidtools/powerstats [Accessed on June 10, 2012]
Walsh, P.S., Metzger, D.A., Higuchi, R., 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques, 10(4): 506–513.
Yuan, G.L., Shen, C.M., Wang, H.D., Liu, W.J., Yang, G., Yan, J.W., Qin, H.X., Xie, T., Ran, H., Yuan, J., et al., 2012. Genetic data provided by 21 autosomal STR loci from Chinese Tujia ethnic group. Mol. Biol. Rep., 39(12): 10265–10271. [doi:10.1007/s11033-012-1903-6]
Zhu, B.F., Wang, Z.Y., Yang, C.H., Li, X.S., Zhu, J., Yang, G., Huang, P., Liu, Y., 2005. Y-chromosomal STR haplotypes in Chinese Uigur ethnic group. Int. J. Legal Med., 119(5):306–309. [doi:10.1007/s00414-005-0549-5]
Zhu, B.F., Yang, G., Shen, C.M., Qin, H.X., Fan, S.L., Deng, L.B., Chen, F., Chen, L.P., Wang, H.D., Wang, Z.Y., et al., 2010. Population genetic analysis of 15 autosomal STR loci in the Russian population of northeastern Inner-Mongolia, China. Mol. Biol. Rep., 37(8):3889–3895. [doi:10.1007/s11033-010-0045-y]
Zhu, B.F., Shen, C.M., Wang, H.D., Yang, G., Yan, J.W., Qin, H.X., Guo, J.X., Huang, J.F., Jing, H., Liu, X.S., 2011. Genetic diversities of 21 non-CODIS autosomal STRs of a Chinese Tibetan ethnic minority group in Lhasa. Int. J. Legal Med., 125(4):581–585. [doi:10.1007/s00414-010-0519-4]
Author information
Authors and Affiliations
Corresponding author
Additional information
Project supported by the Program for New Century Excellent Talents of the Ministry of Education, China (No. NECT-10-0687), the Fundamental Research Funds for the Central University (No. 2011jdgz20), and the Shaanxi Provincial Science and Technology Research and Development Project Fund (No. 2012K16-08-01), China
Rights and permissions
About this article
Cite this article
Wang, Hd., Shen, Cm., Liu, Wj. et al. Allelic frequency distributions of 21 non-combined DNA index system STR loci in a Russian ethnic minority group from Inner Mongolia, China. J. Zhejiang Univ. Sci. B 14, 533–540 (2013). https://doi.org/10.1631/jzus.B1200262
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1631/jzus.B1200262