Journal of Zhejiang University SCIENCE B

, Volume 13, Issue 1, pp 20–28 | Cite as

Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro

  • Yu-li Huang
  • Ruo-feng Qiu
  • Wei-yi Mai
  • Jian Kuang
  • Xiao-yan Cai
  • Yu-gang Dong
  • Yun-zhao Hu
  • Yuan-bin Song
  • An-ping Cai
  • Zhi-gao Jiang
Article

Abstract

Objective

To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs).

Methods

MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5–20 ng/ml. Proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR).

Results

The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA.

Conclusions

IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.

Key words

Mesenchymal stem cells (MSCs) Proliferation Differentiation Insulin-like growth factor-1 (IGF-1) CXC chemokine receptor 4 (CXCR4) Migration 

CLC number

Q291 R542.2 

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Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Yu-li Huang
    • 1
    • 2
  • Ruo-feng Qiu
    • 1
  • Wei-yi Mai
    • 1
  • Jian Kuang
    • 1
  • Xiao-yan Cai
    • 2
  • Yu-gang Dong
    • 1
  • Yun-zhao Hu
    • 2
  • Yuan-bin Song
    • 2
  • An-ping Cai
    • 1
  • Zhi-gao Jiang
    • 1
  1. 1.Department of Cardiologythe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina
  2. 2.Department of Cardiologythe First People’s Hospital of ShundeFoshanChina

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