Age-dependent expression of forkhead box O proteins in the duodenum of rats

  • Pan Huang
  • Zhen-qi Zhou
  • Rui-hua Huang
  • Bo Zhou
  • Quan-wei Wei
  • Fang-xiong Shi
Article

Abstract

The O subfamily of forkhead box (FoxO) proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B (IGF-1/PI3K/PKB) signal pathway. The objective of the present study was to examine the expressions of three members of FoxO proteins, FoxO1, FoxO3a, and FoxO4 in the duodenum of Sprague-Dawley rats at different ages. The result demonstrated that the expression of FoxO4 in rat duodenum showed an age-dependent manner. At Day 21, there were no detectable localization and expression of FoxO4 in the duodenum, while, at Months 2 and 6, localization and expression of FoxO4 were distinct. In addition, FoxO4 staining was primarily concentrated in the cell nuclei of the lamina propria around the intestinal gland of the duodenum in 2-month-old rats, but was not detectable in the same area in 6-month-old rats. Our results showed also that although FoxO3a existed in the cytoplasm of the lamina propria at a low level at the 2- and 6-month marks, it was still not detectable at Day 21. Besides, FoxO1 was not detectable in all parts and stages. Taken together, our findings suggested that the cell-specific and age-dependent expressional patterns of FoxO4 and FoxO3a proteins in the duodenum play some roles in the development and growth performance of the rat duodenum.

Key words

O subfamily of forkhead box (FoxO) Age-dependence Duodenum Rat 

CLC number

Q593 

References

  1. Bao, S., Ouyang, G., Bai, X., Huang, Z., Ma, C., Liu, M., Shao, R., Anderson, R.M., Rich, J.N., Wang, X.F., 2004. Periostin potently promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway. Cancer Cell, 5(4):329–339. [doi:10.1016/S1535-6108(04)00081-9]PubMedCrossRefGoogle Scholar
  2. Brunet, A., Bonni, A., Zigmond, M.J., Lin, M.Z., Juo, P., Hu, L.S., Anderson, M.J., Arden, K.C., Blenis, J., Greenberg, M.E., 1999. Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor. Cell, 96(6):857–868. [doi:10.1016/S0092-8674(00)80595-4]PubMedCrossRefGoogle Scholar
  3. Burgering, B.M., Kops, G.J., 2002. Cell cycle and death control: long live forkheads. Trends Biochem. Sci., 27(7): 352–360. [doi:10.1016/S0968-0004(02)02113-8]PubMedCrossRefGoogle Scholar
  4. Ding, W., Wang, W., Zhou, B., Zhang, W., Huang, P., Shi, F., Taya, K., 2010. Formation of primordial follicles and immunolocalization of PTEN, PKB and FOXO3A proteins in the ovaries of fetal and neonatal pigs. J. Reprod. Dev., 56(1):162–168. [doi:10.1262/jrd.09-094H]PubMedCrossRefGoogle Scholar
  5. Furuyama, T., Yamashita, H., Kitayama, K., Higami, Y., Shimokawa, I., Mori, N., 2002. Effects of aging and caloric restriction on the gene expression of FoxO1, 3, and 4 (FKHR, FKHRL1, and AFX) in the rat skeletal muscles. Microsc. Res. Tech., 59(4):331–334. [doi:10.1002/jemt.10213]PubMedCrossRefGoogle Scholar
  6. Gao, J., Wang, S., Xing, S., 1995. Histological and histochemical observation on the development and differentiation of the mouse duodenum. Acta Anat. Sin., 26(5): 426–431.Google Scholar
  7. Hoekman, M.F., Jacobs, F.M., Smidt, M.P., Burbach, J.P., 2006. Spatial and temporal expression of FoxO transcription factors in the developing and adult murine brain. Gene Expr. Patterns, 6(2):134–140. [doi:10.1016/j.modgep.2005.07.003]PubMedCrossRefGoogle Scholar
  8. Liu, Y., Wang, Y., Shan, T., 2008. The tissue-specific and developmental expression patterns of the forkhead transcription factor FoxO1 gene in pigs. J. Anim. Feed Sci., 17(2):182–190.Google Scholar
  9. Meng, C., Shi, F., Zhou, Z., Huang, R., Liu, G., Watanabe, G., Taya, K., 2007. Cellular localization of inhibin α-subunit, PKB/Akt and FoxO3a proteins in the ovaries of minipigs. J. Reprod. Dev., 53(2):229–236. [doi:10.1262/jrd.18078]PubMedCrossRefGoogle Scholar
  10. Roubenoff, R., Hughes, V.A., 2000. Sarcopenia: current concepts. J. Gerontol. A Biol. Sci. Med. Sci., 55(12): M716–M724. [doi:10.1093/gerona/55.12.M716]PubMedCrossRefGoogle Scholar
  11. Saito, Y., Gopalan, B., Mhashilkar, A.M., Roth, J.A., Chada, S., Zumstein, L., Ramesh, R., 2003. Adenovirus-mediated PTEN treatment combined with caffeine produces a synergistic therapeutic effect in colorectal cancer cells. Cancer Gene Ther., 10(11):803–813. [doi:10.1038/sj.cgt.7700644]PubMedCrossRefGoogle Scholar
  12. Sengupta, A., Molkentin, J.D., Yutzey, K.E., 2009. FoxO transcription factors promote autophagy in cardiomyocytes. J. Biol. Chem., 284(41):28319–28331. [doi:10.1074/jbc.M109.024406]PubMedCrossRefGoogle Scholar
  13. Shi, F., Stewart, R.L.Jr., Perez, E., Chen, J.Y., LaPolt, P.S., 2004. Cell-specific expression and regulation of soluble guanylyl cyclase α1 and β1 subunits in the rat ovary. Biol. Reprod., 70(6):1552–1561. [doi:10.1095/biolreprod.103.025510]PubMedCrossRefGoogle Scholar
  14. Skurk, C., Maatz, H., Kim, H.S., Yang, J., Abid, M.R., Aird, W.C., Walsh, K., 2004. The Akt-regulated forkhead transcription factor FOXO3a controls endothelial cell viability through modulation of the caspase-8 inhibitor FLIP. J. Biol. Chem., 279(2):1513–1525. [doi:10.1074/jbc.M304736200]PubMedCrossRefGoogle Scholar
  15. Tomita, M., Shimokawa, I., Higami, Y., Yanagihara-Outa, K., Kawahara, T., Tanaka, K., Ikeda, T., Shindo, H., 2001. Modulation by dietary restriction in gene expression related to insulin-like growth factor-1 in rat muscle. Aging, 13(4):261–262.Google Scholar
  16. van der Heide, L.P., Hoekman, M.F., Smidt, M.P., 2004. The ins and outs of FoxO shuttling: mechanisms of FoxO translocation and transcriptional regulation. Biochem. J., 380(2):297–309. [doi:10.1042/BJ20040167]CrossRefGoogle Scholar
  17. Zhang, W., Wei, Q., Wang, Z., Ding, W., Wang, W., Shi, F., 2011. Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats. J. Zhejiang Univ.-Sci. B, 12(1):55–64. [doi:10.1631/jzus.B1000174]PubMedCrossRefGoogle Scholar
  18. Zhou, Z., Wang, T., Pan, L., Huang, R., Shi, F., 2007. FoxO4 is the main forkhead transcriptional factor localized in the gastrointestinal tracts of pigs. J. Zhejiang Univ.-Sci. B, 8(1):39–44. [doi:10.1631/jzus.2007.B0039]PubMedCrossRefGoogle Scholar

Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Pan Huang
    • 1
  • Zhen-qi Zhou
    • 1
  • Rui-hua Huang
    • 1
  • Bo Zhou
    • 1
  • Quan-wei Wei
    • 1
  • Fang-xiong Shi
    • 1
  1. 1.College of Animal Science and TechnologyNanjing Agricultural UniversityNanjingChina

Personalised recommendations