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Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita

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Abstract

Seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control. Endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as seed treatment agents against Meloidogyne incognita. Among the 294 isolates screened, 23 significantly reduced galls formed by M. incognita in greenhouse test. The 10 most effective isolates were Fusarium (5), Trichoderma (1), Chaetomium (1), Acremonium (1), Paecilomyces (1), and Phyllosticta (1). Their control efficacies were repeatedly tested and their colonizations as well as in vitro activity against M. incognita were studied. They reduced the number of galls by 24.0%–58.4% in the first screening and 15.6%–44.3% in the repeated test, respectively. Phyllosticta Ph511 and Chaetomium Ch1001 had high colonizations on both the roots and the aboveground parts of cucumber seedlings. Fusarium isolates had colonization preference on the roots, their root colonizations ranging from 20.1% to 47.3% of the total root area. Trichoderma Tr882, Paecilomyces Pa972, and Acremonium Ac985 had low colonizations on both the roots and the aboveground parts. Acremonium Ac985, Chaetomium Ch1001, Paecilomyces Pa972, and Phyllosticta Ph511 produced compounds affecting motility of the second stage juveniles of M. incognita. Based on these results, Chaetomium Ch1001 was considered to have the highest potential as a seed treatment agent for M. incognita biocontrol.

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Authors and Affiliations

  1. Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China

    Xiao-ning Yan & Jing-wu Zheng

  2. Soil-Ecosystem Phytopathology and Nematology, Institute for Resource Conservation and Plant Science (INRES), Department of Plant Health, University of Bonn, Bonn, 53115, Germany

    Richard A. Sikora

  3. Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, China

    Xiao-ning Yan

Authors
  1. Xiao-ning Yan
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  2. Richard A. Sikora
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  3. Jing-wu Zheng
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Correspondence to Jing-wu Zheng.

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Project supported by the German Academic Exchange Agency (DAAD), the China Scholarship Council (CSC), the Special Fund for Agro-scientific Research in the Public Interest (No. nyhezx07-050-6), the Science and Technology Department of Zhejiang Province of China (No. 2008C22062), and the Zhejiang Provincial Natural Science Foundation of China (No. R307396)

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Yan, Xn., Sikora, R.A. & Zheng, Jw. Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita . J. Zhejiang Univ. Sci. B 12, 219–225 (2011). https://doi.org/10.1631/jzus.B1000165

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  • DOI: https://doi.org/10.1631/jzus.B1000165

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