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Journal of Zhejiang University SCIENCE B

, Volume 11, Issue 11, pp 851–861 | Cite as

Transgenic Brassica chinensis plants expressing a bacterial codA gene exhibit enhanced tolerance to extreme temperature and high salinity

Article

Abstract

Transgenic Brassica compestris L. spp. chinensis plants expressing a choline oxidase (codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation. In the transgenic plants, codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (ELISA) examination, immunogold localization, and 1H-nuclear magnetic resonance (1H-NMR). Stress tolerance was evaluated in the T3 plants under extreme temperature and salinity conditions. The plants of transgenic line 1 (L1) showed significantly higher net photosynthetic rate (P n) and P n recovery rate under high (45 °C, 4 h) and low temperature (1 °C, 48 h) treatments, and higher photosynthetic rate under high salinity conditions (100, 200, and 300 mmol/L NaCl, respectively) than the wild-type plants. The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine, which is not found in the wild-type plants. Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L. spp. chinensis could be a potential strategy for improving the plant tolerance to multiple stresses.

Key words

Brassica compestris L. spp. chinensis codA Stress Glycine betaine Net photosynthetic rate (Pn

CLC number

Q943.2 

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Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Department of Agronomy, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina
  2. 2.Shanghai Institute of Plant Physiology and EcologyChinese Academy of ScienceShanghaiChina
  3. 3.School of Resource and Environment ManagementGuizhou College of Finance and EconomicsGuiyangChina

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