Journal of Zhejiang University SCIENCE B

, Volume 11, Issue 3, pp 151–168 | Cite as

Global nutrient profiling by Phenotype MicroArrays: a tool complementing genomic and proteomic studies in conidial fungi

Review

Abstract

Conidial fungi or molds and mildews are widely used in modern biotechnology as producers of antibiotics and other secondary metabolites, industrially important enzymes, chemicals and food. They are also important pathogens of animals including humans and agricultural crops. These various applications and extremely versatile natural phenotypes have led to the constantly growing list of complete genomes which are now available. Functional genomics and proteomics widely exploit the genomic information to study the cell-wide impact of altered genes on the phenotype of an organism and its function. This allows for global analysis of the information flow from DNA to RNA to protein, but it is usually not sufficient for the description of the global phenotype of an organism. More recently, Phenotype MicroArray (PM) technology has been introduced as a tool to characterize the metabolism of a (wild) fungal strain or a mutant. In this article, we review the background of PM applications for fungi and the methodic requirements to obtain reliable results. We also report examples of the versatility of this tool.

Key words

Biolog Phenotype MicroArray Mitosporic fungi Carbon metabolism Trichoderma Aspergillus Biotechnology 

CLC number

Q93 

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© Zhejiang University and Springer Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Research Area of Gene Technology and Applied Biochemistry, Institute of Chemical EngineeringVienna University of TechnologyViennaAustria

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