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Journal of Zhejiang University SCIENCE B

, Volume 11, Issue 10, pp 745–753 | Cite as

Derived vascular endothelial cells induced by mucoepidermoid carcinoma cells: 3-dimensional collagen matrix model

  • Sen Yang
  • Li-juan Guo
  • Qing-hong Gao
  • Ming Xuan
  • Ke Tan
  • Qiang Zhang
  • Yu-ming Wen
  • Chang-mei Wang
  • Xiu-fa Tang
  • Xiao-yi Wang
Article

Abstract

Mucoepidermoid carcinoma undergoes uniquely vigorous angiogenic and neovascularization processes, possibly due to proliferation of vascular endothelial cells (ECs) induced by mucoepidermoid carcinoma cells (MCCs) in their three-dimensional (3D) microenvironment. To date, no studies have dealt with tumor cells and vascular ECs from the same origin of mucoepidermoid carcinoma using the in vitro 3D microenvironment model. In this context, the current research aims to observe neovascularization with mucoepidermoid carcinoma microvascular ECs (MCMECs) conditioned by the microenvironment in the 3D collagen matrix model. We observed the growth of MCMECs purified by immunomagnetic beads and induced by MCCs, and characteristics of tubule-like structures (TLSs) formed by induced MCMECs or non-induced MCMECs. The assessment parameters involved the growth curve, the length, the outer and inner diameters, and the wall thickness of the TLSs, and the cell cycle. Results showed that MCCs induced formation of the TLSs in the 3D collagen matrix model. A statistically significant difference was noted regarding the count of TLSs between the control group and the induction group on the 4th day of culture (t=5.00, P=0.001). The outer and inner diameters (t 1=5.549, P 1=0.000; t 2=10.663, P 2=0.000) and lengths (t=18.035, P=0.000) of the TLSs in the induction group were statistically significant larger than those in the control group. The TLSs were formed at the earlier time in the induction group compared with the control group. It is concluded that MCCs promote growth and migration of MCMECs, and formation of the TLSs. The 3D collagen matrix model with MCMECs induced by MCCs in the current research may be a favorable choice for research on pro-angiogenic factors in progression of mucoepidermoid carcinoma.

Key words

Mucoepidermoid carcinoma Vascular endothelial cells 3-dimensional collagen matrix model 

CLC number

Q37 

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References

  1. Barnes, L., Eveson, J.W., Reichart, P., Sidransky, D., 2005. World Health Organization Classification of Tumours: Pathology and Genetics of Head and Neck Tumours. IARC Press, Lyon, France, p.219–220.Google Scholar
  2. Bian, X.W., 2001. Angiogenesis, therapeutic angiogenesis and antiangiogenesis. Acta Acad. Med. Milit. Tert., 23(3): 249–250 (in Chinese).Google Scholar
  3. Bian, X.W., Chen, J.H., Jiang, X.F., Bai, J.S., Wang, Q.L., Zhang, X., 2004. Angiogenesis as an immunopharmacologic target in inflammation and cancer. Int. Immunopharmacol., 4(12):1537–1547. [doi:10.1016/j.intimp.2004.07.017]CrossRefPubMedGoogle Scholar
  4. Bian, X.W., Jiang, X.F., Chen, J.H., Bai, J.S., Dai, C., Wang, Q.L., Lu, J.Y., Zhao, W., Xin, R., Liu, M.Y., et al., 2006. Increased angiogenic capabilities of endothelial cells from microvessels of malignant human gliomas. Int. Immunopharmacol., 6(1):90–99. [doi:10.1016/j.intimp.2005.08.004]CrossRefPubMedGoogle Scholar
  5. Brat, D.J., van Meir, E.G., 2004. Vaso-occlusive and prothrombotic mechanisms associated with tumor hypoxia, necrosis, and accelerated growth in glioblastoma. Lab. Invest., 84(4):397–405. [doi:10.1038/labinvest.3700070]CrossRefPubMedGoogle Scholar
  6. Folkman, J., 1971. Tumor angiogenesis: therapeutic implications. N. Engl. J. Med., 285(21):1182–1186.CrossRefPubMedGoogle Scholar
  7. Folkman, J., 1996. Fighting cancer by attacking its blood supply. Sci. Am., 275(3):150–154. [doi:10.1038/scientificamerican0996-150]CrossRefPubMedGoogle Scholar
  8. Hao, X., Sun, B., Zhang, S., Zhao, X., 2002. Microarray study of vasculogenic mimicry in bi-directional differentiation malignant tumor. Natl. Med. J. China, 82(19):1298–1302 (in Chinese).Google Scholar
  9. Hendrix, M.J., Seftor, E.A., Meltzer, P.S., Gardner, L.M., Hess, A.R., Kirschmann, D.A., Schatteman, G.C., Seftor, R.E., 2001. Expression and functional significance of VE-cadherin in aggressive human melanoma cells: role in vasculogenic mimicry. PNSA, 98(14):8018–8023. [doi:10.1073/pnas.131209798]CrossRefGoogle Scholar
  10. Hess, A.R., Seftor, E.A., Gardner, L.M., Carles-Kinch, K., Schneider, G.B., Seftor, R.E., Kinch, M.S., Hendrix, M.J., 2001. Molecular regulation of tumor cell vasculogenic mimicry by tyrosine phosphorylation: role of epithelial cell kinase (Eck/EphA2). Cancer Res., 61(8):3250–3255.PubMedGoogle Scholar
  11. Konerding, M.A., Fait, E., Dimitropoulou, C., Malkusch, W., Ferri, C., Giavazzi, R., Coltrini, D., Presta, M., 1998. Impact of fibroblast growth factor-2 on tumor microvascular architecture: a tridimensional morphometric study. Am. J. Pathol., 152(6):1607–1616.PubMedGoogle Scholar
  12. Kuzu, L., Bicknell, R., Harris, A.L., Jones, M., Gatter, K.C., Mason, D.Y., 1992. Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours. J. Clin. Pathol., 45(2):143–148. [doi:10.1136/jcp.45.2.143]CrossRefPubMedGoogle Scholar
  13. Lopes, M.A., Kowalski, L.P., Santos, G.C., de Almeida, O.P., 1999. A clinicopathologic study of 196 intraoral minor salivary gland tumours. J. Oral Pathol. Med., 28(6): 264–267. [doi:10.1016/S0278-2391(00)90075-2]CrossRefPubMedGoogle Scholar
  14. Lorincz, T., Toth, J., Szendroi, M., Timar, J., 2005. Microvascular density of breast cancer in bone metastasis: influence of therapy. Anticancer Res., 25(4):3075–3081.PubMedGoogle Scholar
  15. Miettinen, M., Lindenmayer, A.E., Chaubal, A., 1994. Endothelial cell markers CD31, CD34, and BNH9 antibody to H- and Y-antigens-evaluation of their specificity and sensitivity in the diagnosis of vascular tumors and comparison with von Willebrand factor. Mod. Pathol., 7(1): 82–90.PubMedGoogle Scholar
  16. Parums, D.V., Cordell, J.L., Micklem, K., Heryet, A.R., Gatter, K.C., Mason, D.Y., 1990. JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J. Clin. Pathol., 43(9):752–757. [doi:10.1136/jcp.43.9.752]CrossRefPubMedGoogle Scholar
  17. Shirakawa, K., Kobayashi, H., Heike, Y., Kawamoto, S., Brechbiel, M.W., Kasumi, F., Iwanaga, T., Konishi, F., Terada, M., Wakasugi, H., 2002a. Hemodynamics in vasculogenic mimicry and angiogenesis of inflammatory breast cancer xenograft. Cancer Res., 62(2):560–566.PubMedGoogle Scholar
  18. Shirakawa, K., Wakasugi, H., Heike, Y., Watanabe, I., Yamada, S., Saito, K., Konishi, F., 2002b. Vasculogenic mimicry and pseudo-comedo formation in breast cancer. Int. J. Cancer, 99(6):821–828. [doi:10.1002/ijc.10423]CrossRefPubMedGoogle Scholar
  19. Vargas, P.A., Gerhard, R., Araújo Filho, V.J., de Castro, I.V., 2002. Salivary gland tumors in a Brazilian population: a retrospective study of 124 cases. Rev. Hosp. Clin. Fac. Med. Sao. Paulo., 57(6):271–276.PubMedGoogle Scholar
  20. Waldron, C.A., el-Mofty, S.K., Gnepp, D.R., 1988. Tumors of the intraoral minor salivary glands: a demographic and histologic study of 426 cases. Oral Surg. Oral Med. Oral Pathol., 66(3):323–333. [doi:10.1016/0030-4220(88)90240-X]CrossRefPubMedGoogle Scholar
  21. Yang, S., Wang, X.Y., Guo, L.J., Tang, X.F., Gao, Q.H., Xuan, M., Zhang, Q., Tan, K., Wen, Y.M., Wang, C.M., et al., 2008. Correlation between the expression of thrombospondin-1 and neovascularization in mucoepidermoid carcinoma. Chin. Med. J., 121(19):1875–1881.PubMedGoogle Scholar
  22. Zeng, Q., Li, S., Chepeha, D.B., Giordano, T.J., Li, J., Zhang, H., Polverini, P., Nor, J., Kitajewski, J., Wang, C., 2005. Crosstalk between tumor and endothelial cells promotes tumor angiogenesis by MAPK activation of Notch signaling. Cancer Cell, 8(1):13–23. [doi:10.1016/j.ccr.2005.06.004]CrossRefPubMedGoogle Scholar

Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Sen Yang
    • 1
    • 2
  • Li-juan Guo
    • 1
  • Qing-hong Gao
    • 2
  • Ming Xuan
    • 2
  • Ke Tan
    • 3
  • Qiang Zhang
    • 3
  • Yu-ming Wen
    • 2
  • Chang-mei Wang
    • 2
  • Xiu-fa Tang
    • 2
  • Xiao-yi Wang
    • 2
  1. 1.State Key Laboratory of Oral DiseasesSichuan UniversityChengduChina
  2. 2.Department of Head and Neck Tumor Surgery, West China College of StomatologySichuan UniversityChengduChina
  3. 3.Department of Health Statistics, West China College of Public HealthSichuan UniversityChengduChina

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