Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture
- 68 Downloads
The adhesion of monocytes to the endothelium following accumulation of low-density lipoprotein (LDL) in subendothelial spaces is an important step in the development of intimal hyperplasia in arterially implanted vein grafts and atherosclerosis in both animals and humans. However, it is not well known how serum factors affect the adhesion of monocytes.
We have studied the effect of fetal calf serum (FCS), which we considered a source of LDL, on the adhesion of monocytes to endothelial cells (ECs) by using human monocytic THP-1 cells and both a monolayer of cultured bovine aortic endothelial cells (EC monoculture) and a co-culture with bovine aortic smooth muscle cells (EC-SMC co-culture).
It was found that the addition of FCS to the medium greatly affected the adhesion of THP-1 cells, and the higher the concentration of FCS in the medium, the greater the adhesion of THP-1 cells to endothelial cells. Adhesion of THP-1 cells to an EC-SMC co-culture was approximately twofold greater than that to an EC monoculture, and after adhering to endothelial cells, many THP-1 cells transmigrated into the layer of smooth muscle cells.
The results suggest that the elevation of the LDL (cholesterol) level in blood provides a favorable condition for the development of intimal hyperplasia and atherosclerosis by promoting the adhesion of monocytes to the endothelium and their subsequent migration into subendothelial spaces.
Key wordsEndothelial cells (ECs) Smooth muscle cells (SMCs) Monocyte THP-1 cells Low-density lipoprotein (LDL) Adhesion Transendothelial migration Serum concentration
Unable to display preview. Download preview PDF.
- Cai, Q.J., Lanting, L., Natarajan, R., 2004. Interaction of monocytes with vascular smooth muscle cells regulate monocyte survival and differentiation through distant pathways. Atheriosclerosis Thrombosis and Vascular Biology, 24(12):2263–2270. [doi:10.1161/01.ATV.0000146552.16943.5e]CrossRefGoogle Scholar
- Cushing, S.D., Berliner, J.A., Valente, A.J., Territo, M.C., Navab, M., Parhami, F., Gerrity, R., Schwartz, C.J., Fogelman, A.M., 1990. Minimally modified low density lipoprotein induces monocyte chemotactic protein 1 in human endothelial cells and smooth muscle cells. Proc. Natl. Acad. Sci. USA, 87(13):5134–5138. [doi:10.1073/pnas.87.13.5134]PubMedCrossRefGoogle Scholar
- Kinard, F., Jaworshi, K., Sergent-Engelen, T., Goldstein, D., van Veldhoven, P.P., Holvoet, P., Trouet, A., Schneider, Y.J., Remacle, C., 2001. Smooth muscle cells influence monocyte response to LDL as well as their adhesion and transmigration in a coculture model of the arterial wall. J. Vasc. Res., 38(5):479–491. [doi:10.1159/000051081]PubMedCrossRefGoogle Scholar
- Michison, M.J., Carpenter, K.L.H., Ball, R.Y., 1990. The Role of Macrophages in Human Atherosclerosis. In: Glagov, S., Newman, W.P., Schaffer, S.A. (Eds.), Pathobiology of the Human Atherosclerotic Plaque. Springer-Verlag, New York, p.121–128.Google Scholar