Journal of Zhejiang University SCIENCE B

, Volume 9, Issue 4, pp 291–298 | Cite as

Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia

  • Xiao-li Hou
  • Han-liang Jiang
  • Qing-yi Cao
  • Li-ying Zhao
  • Barbara J. Chang
  • Zhi Chen


The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.

Key words

Oligonucleotide array Bacteremia 23S rRNA Multiplexed detection 

CLC number



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Copyright information

© Zhejiang University Press 2008

Authors and Affiliations

  • Xiao-li Hou
    • 1
  • Han-liang Jiang
    • 1
  • Qing-yi Cao
    • 1
  • Li-ying Zhao
    • 1
  • Barbara J. Chang
    • 2
  • Zhi Chen
    • 1
  1. 1.Institute of Infectious Diseases, the First Affiliated Hospital, School of MedicineZhejiang UniversityHangzhouChina
  2. 2.Department of Microbiology and Immunology, School of Biomedical, Biomolecular and Chemical SciencesUniversity of Western AustraliaNedlandsAustralia

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