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Journal of Zhejiang University SCIENCE B

, Volume 9, Issue 2, pp 132–140 | Cite as

Molecular and functional comparisons of the vacuolar Na+/H+ exchangers originated from glycophytic and halophytic species

  • Jin-yao Li
  • Xiao-wei He
  • Li Xu
  • Jie Zhou
  • Ping Wu
  • Hui-xia Shou
  • Fu-chun Zhang
Article

Abstract

A novel vacuolar Na+/H+ exchanger, CgNHX1, was cloned from a halophytic species Chenopodium glaucum by using reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technique. Sequence alignment and phylogenetic analysis of 22 NHX genes from GenBank as well as the new CgNHX1 gene indicate that NHX genes shared a great degree of similarity, regardless of their glycophytic or halophytic origin. Expression of the CgNHX1 gene was induced by NaCl and peaked at 400 mmol/L NaCl. Overexpression of NHX1 genes in rice enhanced their tolerance to salt stress. However, there is no significant difference in salt tolerance among the transgenic rice plants overexpressing the NHX1 genes from either glycophytic or halophytic species. The Na+ content of both the wild type (WT) and transgenic plants increased when exposed to 50 and 100 mmol/L NaCl, and the Na+ concentration in transgenic plants was marginally higher than that of WT. Our data demonstrate that the overexpression of the NHX1 gene from either glycophytic or halophytic species resulted in the enhanced tolerance to salt stress at a similar level, suggesting that NHX gene per se might not be the reason accounting for the difference in salt tolerance between glycophytes and halophytes.

Key words

NHX gene Rice transformation Salt stress Tolerance 

Document code

CLC number

Q3 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Life SciencesZhejiang UniversityHangzhouChina
  2. 2.Key Laboratory of Molecular Biology, College of Life Science and TechnologyXinjiang UniversityUrumqiChina

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