Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis
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Performance of biological phosphorus removal in the oxic-settling-anaerobic (OSA) process was investigated. Cell staining and fluorescent in situ hybridization (FISH) were used to analyze characteristics and microbial community of sludge. Experimental results showed that phosphorus removal efficiency was near 60% and the amount of biological phosphorus accumulation in aerobic sludge of the OSA system was up to 26.9 mg/g. Biological phosphorus removal efficiency was partially inhibited by carbon sources in the continuous OSA system. Contrasted to the OSA system, biological phosphorus removal efficiency was enhanced by 14% and the average total phosphorus (TP) contents of aerobic sludge were increased by 0.36 mg/g when sufficient carbon sources were supplied in batch experiments. Staining methods indicated that about 35% of microorganisms had typical characteristics of phosphorus accumulating organisms (PAOs). FISH analysis demonstrated that PAOMIX-binding bacteria were predominant microbial communities in the OSA system, which accounted for around 28% of total bacteria.
KeywordsExcess sludge reduction Biological phosphorus removal Phosphate accumulating organisms (PAOs) DAPI (4′,6′-diamidino-2-phenyl indol dihydrochloride) Fluorescent in situ hybridization (FISH)
CLC numberX703.1 DQ89
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- APHA (American Public Health Association/American Water Works Association/Water Environment Federation), 1995. Standard Methods for the Examination of Water and Wastewater. Washington, DC, USA.Google Scholar
- Bond, P.L., Erhart, R., Wagner, M., Keller, J., Blackall, L.L., 1999. Identification of some of the major groups of bacteria in efficient and nonefficient biological phosphorus removal activated sludge systems. Appl. Environ. Microbiol., 65(9):4077–4084.Google Scholar
- Crocetti, G.R., Hugenholtz, P., Bond, P.L., Schuler, A., Keller, J., Jenkins, D., Blackall, L.L., 2000. Identification of polyphosphate accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Appl. Environ. Microbiol., 66(3):1175–1182. [doi:10.1128/AEM.66.3.1175-1182.2000]CrossRefGoogle Scholar
- Kong, Y., Nielsen, J.L., Nielsen, P.H., 2005. Identity and ecophysiology of uncultured actinobacterial polyphosphate-accumulating organisms in full-scale enhanced biological phosphorus removal plants. Appl. Environ. Microbiol., 71(7):4076–4085. [doi:10.1128/AEM.71.7.4076-4085.2005]CrossRefGoogle Scholar
- Murray, R.G.E., Doetsch, R.N., Robinow, C.F., 1994. Determinative and Cytological Light Microscopy. In: Gehardt, P., Murray, R.G.E., Wood, W.A., Krieg, N.R. (Eds.), Methods for General and Molecular Bacteriology. American Society for Microbiology. Washington, DC, p.21–41.Google Scholar
- Tchobanoglous, G., Burton, F.L., Stensel, H.D., 2003. Wastewater Engineering Treatment and Reuse. Tsinghua University Publishing House, Beijing, p.565, 805–806.Google Scholar
- Wang, J.F., Jin, W.B., Zhao, Q.L., Liu, Z.G., Lin, J.K., 2007. Performance of treating wastewater and anti-impact in oxic-settling-anoxic (OSA) process for minimization of excess sludge. Environ. Sci., 28(11):2488–2493 (in Chinese).Google Scholar