Journal of Zhejiang University SCIENCE B

, Volume 8, Issue 7, pp 453–457 | Cite as

Alterations in seedling vigour and antioxidant enzyme activities in Catharanthus roseus under seed priming with native diazotrophs

  • Karthikeyan B. 
  • Jaleel C. A. 
  • Gopi R. 
  • Deiveekasundaram M. 
Science Letters


An experiment was conducted on Catharanthus roseus to study the effect of seed treatments with native diazotrophs on its seedling growth and antioxidant enzyme activities. The treatments had significant influence on various seedling parameters. There is no significant influence on dry matter production with the diazotrophs, Azospirillum and Azotobacter. However, the vital seedling parameters such as germination percentage and vigour index were improved. Azotobacter treatment influenced maximum of 50% germination, whereas Azospirillum and Azotobacter were on par with C. roseus with respect to their vigour index. There was significant difference in the population of total diazotrophs. Azospirillum and Azotobacter between rhizosphere and non-rhizosphere soils of C. roseus had the same trend and were observed at various locations of the study. The activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) were increased to a significant extent due to the treatment with diazotrophs.

Key words

Rhizosphere Non-rhizosphere Azospirillum Azotobacter Antioxidant enzyme Catharanthus roseus 

CLC number



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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Karthikeyan B. 
    • 1
  • Jaleel C. A. 
    • 2
  • Gopi R. 
    • 2
  • Deiveekasundaram M. 
    • 1
  1. 1.Department of Microbiology, Faculty of AgricultureAnnamalai UniversityAnnamalainagarIndia
  2. 2.Stress Physiology Lab, Department of BotanyAnnamalai UniversityAnnamalainagarIndia

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