Journal of Zhejiang University SCIENCE B

, Volume 8, Issue 4, pp 272–276 | Cite as

Capillary gas chromatographic determination of dimethachlon residues in fresh tobacco leaves and cut-tobacco



Simple procedures for extraction and chromatographic determination of dimethachlon residues in fresh tobacco leaves and cut-tobacco are described. The determination was carried out by capillary gas chromatography (GC) with electron capture detection (ECD) and confirmed by GC-MS. The mean recoveries and relative standard deviation (RSD) were 93.2%∼112.9% and 3.5%∼6.7%, respectively at levels ranging from 0.01 to 0.1 mg/kg. The limit of determination was 0.001 mg/kg. Tobacco samples in routine check were successfully analyzed using the proposed method.

Key words

Dimethachlon residues Tobacco Gas chromatography (GC) 

CLC number



Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chen, A.D., Yan, W.Z., Yang, J.L., 2001. Measurement of tobacco losses of quality and output caused by tobacco spot disease. Chin. J. Plant Prot., 27(6):11–13 (in Chinese).Google Scholar
  2. Huang, B.Y., Pan, C.P., Wang, Y.R., Cao, Z., Zhang, W., Li, W.M., Jiang, S.R., 2006. Rapid determination of pesticide multiresidues in vegetables by GC/MS and compensation by matrix effect with protection. Chem. J. Chin. Univ., 27(2):227–232 (in Chinese).Google Scholar
  3. Jánská, M., Lehotay, S.J., Mastovská, K., Hajslová, J., Alon, T., Amirav, A., 2006. A simple and inexpensive “solvent in silicone tube extraction” approach and its evaluation in the gas chromatographic analysis of pesticides in fruits and vegetables. J. Sep. Sci., 29(1):66–80. [doi:10.1002/jssc.200500237]PubMedCrossRefGoogle Scholar
  4. Lehotay, S.J., Mastovska, K., Lightfield, A.R., 2005. Use of buffering and other means to improve results of problematic pesticides in a fast and easy method for residue analysis of fruits and vegetables. J. AOAC Int., 88(2):615–629.PubMedGoogle Scholar
  5. Li, Y.J., Chen, H.M., Zhou, L., 2003. Experiment on mixed pesticides for controlling tobacco brown spot and Myzus Persicae (Sulzer). Chin. J. Tobacco Sci. Technol./Dis. Pest Control, 10(2):45–47 (in Chinese).Google Scholar
  6. Ma, J., Zheng, R., Xu, L., Wang, S., 2002. Differential sensitivity of two green algae, Scenedesmus obliqnus and Chlorella pyrenoidosa, to 12 pesticides. Ecotoxicol. Environ. Saf., 52(1):57–61. [doi:10.1006/eesa.2002.2146]PubMedCrossRefGoogle Scholar
  7. Mastovska, K., Lehotay, S.J., 2004. Evaluation of common organic solvents for gas chromatographic analysis and stability of multiclass pesticide residues. J. Chromatogr. A, 1040(2):259–272. [doi:10.1016/j.chroma.2004.04.017]PubMedCrossRefGoogle Scholar
  8. Mastovska, K., Hajslova, J., Lehotay, S.J., 2004. Ruggedness and other performance characteristics of low-pressure gas chromatography-mass spectrometry for the fast analysis of multiple pesticide residues in food crops. J. Chromatogr. A, 1054(1–2):335–349. [doi:10.1016/j.chroma.2004.08.061]PubMedCrossRefGoogle Scholar
  9. Shi, J.H., 1996. Gas chromatographic determinate the pesticide of dimethachlon. Chin. J. Pest, 35(6):23–26 (in Chinese).Google Scholar
  10. Shi, Z.Q., Zhou, M.G., Ye, Z.Y., 2000. Resisitance of Sclerotinaia Sclerotiorum to carbendazim and dimethachlon. Chin. J. Oil Crop Sci., 22(4):54–57 (in Chinese).Google Scholar
  11. Wang, H.M., Zhang, Z.M., 1994. A New Pesticide Manual. Agricultural Press, Beijing, China, p.362–363 (in Chinese).Google Scholar
  12. Zhang, X.L., Sun, X.M., Zhang, G.F., 2003. Preliminary report on the mentoring of the resistance of Sclerotinia libertinia to carbendazim and its internal management. Chin. J. Pest. Sci. Adm., 24(6):18–22 (in Chinese).Google Scholar
  13. Zhang, J.J., Cao, Y.Z., Li, X.M., Li, Z.Y., Wu, Y.P., Guo, T.T., 2006. Simultaneous determination of 405 pesticide residues in grain by accelerated solvent extraction then gas chromatography-mass spectrometry or liquid chromatography-tandem mass spectrometry. Anal. Bioanal. Chem., 384(5):1366–1408.PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Supervision and Testing Center for Farm Product Quality, Ministry of Agriculture (Kunming)Yunnan Academy of Agriculture ScienceKunmingChina
  2. 2.Kunming Institute of PhysicsKunmingChina

Personalised recommendations