Characteristics of transgenic tomatoes antisensed for the ethylene receptor genes LeERT1 and LeERT2
- 29 Downloads
Two stable transformed lines containing antisense LeERT1 or LeERT2 sequences and their hybridized line were investigated to determine the effect of LeERT1 and LeERT2 specificity in the ethylene receptor family in tomato (Lycopersicon esculentum Mill.) on ethylene signaling. The transgenic line ale1 containing antisense LeERT1 displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, and accelerated abscission of petiole explant and senescence of flower explant, compared with its wild type B1. The transgenic line ale2 containing antisense LeERT2 also exhibited shorter hypocotyls and slightly accelerated abscission. The phenotypes of cross line dale of LeERT1 and LeERT2 were close to ale1 in many aspects. These results suggested that LeERT1 probably plays a relatively important role in ethylene signaling of tomato growth and development.
Key wordsAntisense transformation Ethylene receptor Ethylene response Tomato
Unable to display preview. Download preview PDF.
- Ciardi, J., Tieman, D., Jones, J., Klee, H.J., 2001. Reduced expression of the tomato ethylene receptor LeERT4 enhances the hypersensitive response to Xanthomonas campestris pv. vesicatoria. Mol. Plant Microbe Interactions, 14(4):487–495.Google Scholar
- Tieman, D.M., Taylor, M.G., Ciardi, J.A., Klee, H.J., 2000. The tomato ethylene receptors NR and LeERT4 are negative regulators of ethylene response and exhibit functional compensation within a multigene family. Proc. Nat. Acad. Sci. USA, 97(10):5663–5668. [doi:10.1073/pnas.090550597]PubMedCrossRefGoogle Scholar
- Wang, H., Qi, M.Q., Cutler, A.C., 1993. A simple method of preparing plant samples for PCR. Nucleic Acid. Research, 21(17):4153–4154.Google Scholar
- Whitelaw, C.A., Lyssenko, N.N., Chen, L., Zhou, D., Mattoo, A.K., Tucker, M.L., 2002. Delayed abscission and shorter internodes correlate with a reduction in the ethylene receptor LeERT1 transcript in transgenic tomato. Plant Physiol., 128(3):978–987. [doi:10.1104/pp.010782]PubMedCrossRefGoogle Scholar
- Zheng, T.S., Ying, T.J., Zeng, G.W., He, G.Q., 2001. Expression of ethylene receptor gene LeERT2 in tomato mutant Epi and construction of its antisense expression vector. J. Zhejiang Univ. (Agr. and Life Sci.), 27(2):156–160 (in Chinese).Google Scholar
- Zhou, D., Mattoo, A.K., Tucker, M.L., 1996b. Molecular cloning of a tomato cDNA (accession No. U47279) encoding an ethylene receptor. Plant Physiol., 110(4):1435.Google Scholar