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Study of cellulolytic soil fungi and two nova species and new medium

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Abstract

This study is aimed at identifying and determining the percentage of occurrence frequency of cellulose decomposing soil fungi. The soil samples were inoculated into culture plates prepared in Sabouraud medium under sterilized conditions and incubated at 30 °C for 4 to 7 d. The identified fungal species were incubated in self-designed cellulose medium for testing their cellulolytic ability. Forty-two species, including 2 nova species, representing sixteen genera showed growth and sporulation in the cellulose medium. Most of the isolated species were from genus Aspergillus and Penicillium. Aspergillus niger and Mucor hiemalis showed highest occurrence frequency (45% and 36% respectively), as these species were collected from about 80% of soil samples. Being agar free and cheaper, the new fungal medium designed showed results equivalent to Sabouraud medium.

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References

  • Ali-Shtayeh, M.S., Arda, H.M., Hassouna, M., Shaheen, S.F., 1989. Keratinophilic fungi on sheep hairs from West Bank of Jordon. Mycopathologia, 106(2):95–101. [doi:10.1007/BF00437087]

    Article  PubMed  CAS  Google Scholar 

  • Ander, P., Daniel, G., Pettersson, B., Westermark, V., 1996. Possible applications of cellobiose oxidizing and other flavine dinucleotide enzymes in the pulp and paper industry. ACS Symp. Ser., 655:297–307.

    CAS  Google Scholar 

  • Ariunaa, J., Temuulen, G., 2001. Distribution of Cellulolytic Fungi in Soil of Mongolia. Scientific Journal “Biology” Mongolian National University, No. 10, Ulaanbaatar, p.71–77 (in Russian).

  • Baig, M.M., Mane, V.P., More, D.R., Shinde, L.P., Baig, M.I., 2003. Utilization of banana agricultural waste: production of cellulases by soil fungi. J. Environ. Biol., 24(2):173–176.

    PubMed  CAS  Google Scholar 

  • Balasubramanian, A., Bagyaraj, D.J., Rangaswami, G., 1970. Studies of the influence of foliar application of chemicals on the micro flora and certain enzyme activities in the rhizosphere of Eleusine coracane Gaertn. Plant and Soil, 32(1–3):198–206. [doi:10.1007/BF01372858]

    Article  CAS  Google Scholar 

  • Bergquist, P., Te’o, V., Gibbs, M., Curach, N., Nevalainen, K., 2003. Recombinant Bleaching Enzymes from Thermophiles Expressed in Fungal Hosts. In: Mansfield, S.D., Saddler, J.N., (Eds.), Applications of Enzymes to Lignocellulosics. Amer. Chem. Soc. Symposium Series, 855, p.435–445.

  • Brazauskiene, I., 1998. Fungal disease of faba bean and the efficiency of fungicides. Zemdirbyste. Mokslo. Darbai., 62:166–174.

    Google Scholar 

  • Chand, P., Aruna, A., Maqsood, A.M., Rao, L.V., 2005. Novel mutation method for increased cellulase production. Journal of Applied Microbiology, 98(2):318. [doi:10.1111/j.1365-2672.2004.02453.x]

    Article  PubMed  CAS  Google Scholar 

  • Cowling, E.B., 1958. A Review Literature on the Enzymatic Degradation of Wood and Cellulose. USDA Forest Service Report, No. 2116.

  • Difco Laboratories, 1972. Difco Manual, 9th Ed. Difco Laboratories, Michigan.

    Google Scholar 

  • Eggins, H.O.W., Pugh, G.J.F., 1962. Isolation of cellulose decomposing fungi from soil. Nature London, 193:94–95.

    Google Scholar 

  • El-Said, A.H.M., 2001. Mycotoxins and invertase enzyme of the mycoflora of molasses in upper Egypt. Pakistan Journal of Biological Sciences, 4(11):1386–1389.

    Article  Google Scholar 

  • Fenice, M., Selbmann, L., Zucconi, L., Onofri, S., 1997. Production of extracellular enzymes by Antarctic fungal strains. Polar Biology, 17(3):275–280. [doi:10.1007/s003000050132]

    Article  Google Scholar 

  • Fenice, M., Selbmann, L., Di Giambattista, R., Federici, F., 1998. Chitinolytic activity at low temperature of an Antarctic strain (A3) of Verticillium lecanii. Research in Microbiology, 149(4):289–300. [doi:10.1016/S0923-2508(98)80304-5]

    Article  PubMed  CAS  Google Scholar 

  • Garrett, S.D., 1963. Soil Fungi and Soil Fertility. Pergamon Press, Oxford, p.1–165.

    Google Scholar 

  • Gascoigne, J.A., Gascoigne, M.M., 1958. Biological Degradation of Cellulose. Butter Worths Publ. Co., London, p.231.

    Google Scholar 

  • Ghahfarokhi, M.S., Fazal, A., Lotfi, A., Abyaneh, M.R., 2004. Cellobiose dehydrogenase production by the genus cladosporium. Iran. Biomed. J., 8(2):107–111.

    Google Scholar 

  • Gilman, J.C., 1971. A Manual of Soil Fungi, 2nd Ed. Iowa State College Press, Ames, Iowa, p.450.

    Google Scholar 

  • Gochenaur, S.E., 1975. Distributional patterns of mesophilous and thermophilous microfungi in two Bahamian soils. Mycopathologia, 57(3):155–164. [doi:10.1007/BF00551422]

    Article  PubMed  CAS  Google Scholar 

  • Gupta, J.K., Soni, S.K., 2000. Industrial applications of enzymes. J. Pb. Acad. Sci., 2:241–245.

    Google Scholar 

  • Gupta, J.K., Shirkot, C.K., Dhawan, S., 1981. Isolation and mutation of cellulolytic fungi. Acta Microbiol. Acad. Sci. Hung, 28(1):31–36.

    PubMed  CAS  Google Scholar 

  • Henriksson, G., Johansson, G., Pettersson, G., 2000. A critical review of cellobiose dehydrogenases. J. Biotechnol., 78(2):93–113. [doi:10.1016/S0168-1656(00)00206-6]

    Article  PubMed  CAS  Google Scholar 

  • Kelman, R., 1967. Sourcebook of Laboratory Exercises in Plant Pathology. Sourcebook Committee of America Phytopathological Society. W.H. Freman and Co., San Francisco, p.180.

    Google Scholar 

  • Lebedeva, E., Nazarenko, A.V., Vedenyapina, E.G., 1999. Soil micromycetes in the area of “Severstal” Metallurgical Works (Cherepovets, Russia). Botanica Lithuanica, Suppl. 3:107–110.

  • Lilly, V.G., Barnett, H.L., 1951. Physiology of Fungi. Mc Graw-Hill Book Co., New York, p.218.

    Google Scholar 

  • Malik, K.A., Sandhu, G.R., 1973. Decomposition of organic matter by fungi in saline soils. Mycopathol. Mycol. Appl., 50(4):339–347. [doi:10.1007/BF02050034]

    Article  PubMed  CAS  Google Scholar 

  • Mehta, V., Gupta, J.K., 1991. Biotechnology in the pulp and paper industry. Research and Industry, 36:161–167.

    CAS  Google Scholar 

  • Mehta, V., Gupta, J.K., 1992. Biobleaching of Eucalyptus Kraft Pulp with Phaenero Chaete Chrysosporium and its Effect on Paper Properties. Tappi, USA, p.151–152.

    Google Scholar 

  • Moustafa, A.F., 1975. Osmophilous fungi in the salt of Kuwait. Can. J. Microbiol., 21(10):1573–1580.

    Article  PubMed  CAS  Google Scholar 

  • Nasser, L.A., 2003. Distribution of zoosporic and terrestrial fungi in accumulated rainfall water in Abha, south western region, Saudi Arabia. Online Journal of Biological Sciences, 3(9):843–853.

    Google Scholar 

  • Nevalainen, H., Penttilä, M., 2003. Molecular Biology of Cellulolytic Fungi. In: Kück, U. (Ed.), The Mycota, Vol. II, Genetics and Biotechnology, 2nd Ed. Springer Verlag, Berlin, Heidelberg.

    Google Scholar 

  • Nevalainen, H., Te’o, V.S.J., 2003. Enzyme Production in Industrial Fungi—Role of Molecular Genetics. In: Arora, D.K. (Ed.), Applied Mycology and Biotechnology, Vol. 3, Fungal Genomics. Elsevier Science.

  • Norenko, L.N.N., Ariunaa, J., Morozova, E.S., Rabinovich, M.L., 1994. Cyclic Utilization of Lignocellulosics. 8th E.C. on Biomass for Energy, Environment, Agriculture and Industry. Vienna, Austria, p.1337–1342.

  • Ortuno, A., Noguera, J., Hernansaez, A., Armero, T., 1977. Phosphoric metabolism of Aspergillus niger in calcareous and saline soils. Microbiol. Esp., 30–31:101–112.

    PubMed  Google Scholar 

  • Radwan, S.S., El-Essawy, A.A., Helal, G.A., 1984. Salinity-loving fungi in Egyptian soils. I. Numbers, identities, and haplophilism. Zentralbl Mikrobiol., 139(6):435–440.

    PubMed  CAS  Google Scholar 

  • Redman, R.S., Litvintseva, A., Sheehan, K.B., Henson, J.M., Rodriguez, R.J., 1999. Fungi from geothermal soils in yellowstone national park. Applied and Environmental Microbiology, 65(12):5193–5197.

    PubMed  CAS  Google Scholar 

  • Robinson, C.H., 2001. Cold adaptation in Arctic and Antarctic fungi. New Phytologist., 151(2):341–353. [doi:10.1046/j.1469-8137.2001.00177.x]

    Article  CAS  Google Scholar 

  • Robinson, P.M., Morris, G.M., 1984. Tolerance of hyphae of Fusarium oxysporum f.sp. lycopersici to low temperature. Transactions of the British Mycological Society, 83:569–573.

    Article  Google Scholar 

  • Robinson, C.H., Fisher, P.J., Sutton, B.C., 1998. Fungal biodiversity in dead leaves of fertilised plants of Dryas octopetala from a high Arctic site. Mycological Research, 102(5):573–576. [doi:10.1017/S0953756297005492]

    Article  Google Scholar 

  • Shalaby, A.M., Rizk, M.A., Moussa, T.A.A., 2002. Effect of igran on the rhizosphere mycoflora of vicia faba plants grown in soils infested with orabanche crenata and amended with rhizobium leguminosarum. Pakistan Journal of Biological Sciences, 5(5):517–520.

    Article  Google Scholar 

  • Shimosaka, M., Kumehara, M., Zhang, X.Y., Nogawa, M., Okazaki, M., 1996. Cloning and characterization of a chitosanase gene from the plant pathogenic fungus Fusarium solani. J. Ferment. Bioeng., 82(5):426–431. [doi:10.1016/S0922-338X(97)86977-2]

    Article  CAS  Google Scholar 

  • Siu, R.G.H., 1951. Microbial Decomposition of Cellulose. Reihd. Publ. Corp., New York, p.531.

    Google Scholar 

  • Steiner, J., Socha, C., Eyzaguirre, J., 1994. Culture conditions for enhanced cellulase production by a native strain of Penicillium purpurogenum. World J. Microbiol. and Biotech., 10(3):280–284. [doi:10.1007/BF00414863]

    Article  CAS  Google Scholar 

  • Stevens, R.B., 1947. Mycology Guidebook. University of Washington Press, Seattle, p.532.

    Google Scholar 

  • Szakäcs-Dobozi, M., Szakäcs, G., Meyer, D., Klappach, G., 1985. Enhancement of enzymatic degradation of cellulose by application of mixed enzyme systems of different fungal origin. Acta Biotechnologica, 5(1):27–33. [doi:10.1002/abio.370050108]

    Article  Google Scholar 

  • Taiwo, L.B., Oso, B.A., 1997. The influence of some pesticides on soil micro flora in relation to changes in nutrient levels, rock phosphate solubilization and P release under laboratory conditions. Agric. Ecosys. Env., 65(1):59–68. [doi:10.1016/S0167-8809(97)00044-3]

    Article  CAS  Google Scholar 

  • Wainwright, M., 1995. An Introduction to Fungal Biotechnology. Wiley, Chichester.

    Google Scholar 

  • Warcup, J.H., 1950. The soil-plate method for isolation of fungi from the soil. Nature, 166:117.

    PubMed  CAS  Google Scholar 

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Khalid, M., Yang, Wj., Kishwar, N. et al. Study of cellulolytic soil fungi and two nova species and new medium. J. Zhejiang Univ. - Sci. B 7, 459–466 (2006). https://doi.org/10.1631/jzus.2006.B0459

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