Journal of Zhejiang University SCIENCE B

, Volume 7, Issue 6, pp 459–466 | Cite as

Study of cellulolytic soil fungi and two nova species and new medium

  • Khalid Mahmood 
  • Yang Wei-jun 
  • Kishwar Nazir 
  • Rajput Zahid Iqbal 
  • Arijo Abdullah G. 


This study is aimed at identifying and determining the percentage of occurrence frequency of cellulose decomposing soil fungi. The soil samples were inoculated into culture plates prepared in Sabouraud medium under sterilized conditions and incubated at 30 °C for 4 to 7 d. The identified fungal species were incubated in self-designed cellulose medium for testing their cellulolytic ability. Forty-two species, including 2 nova species, representing sixteen genera showed growth and sporulation in the cellulose medium. Most of the isolated species were from genus Aspergillus and Penicillium. Aspergillus niger and Mucor hiemalis showed highest occurrence frequency (45% and 36% respectively), as these species were collected from about 80% of soil samples. Being agar free and cheaper, the new fungal medium designed showed results equivalent to Sabouraud medium.

Key words

Cellulolytic soil fungi Cellulose medium Nova species Taxonomy 

CLC number



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Copyright information

© Zhejiang University 2006

Authors and Affiliations

  • Khalid Mahmood 
    • 1
    • 2
  • Yang Wei-jun 
    • 2
  • Kishwar Nazir 
    • 3
  • Rajput Zahid Iqbal 
    • 4
  • Arijo Abdullah G. 
    • 5
  1. 1.Government CollegeRawalpindiPakistan
  2. 2.School of Life SciencesZhejiang UniversityHangzhouChina
  3. 3.Natural History MuseumIslamabadPakistan
  4. 4.R & D Section, Directorate of Animal Husbandry SindhHyderabadPakistan
  5. 5.Department of ParasitologySindh Agriculture UniversityTando JamPakistan

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