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Journal of Zhejiang University SCIENCE B

, Volume 7, Issue 4, pp 272–278 | Cite as

Intra-specific genetic relationship analyses of Elaeagnus angustifolia based on RP-HPLC biochemical markers

  • Wang Qiang 
  • Ruan Xiao 
  • Huang Jun-hua 
  • Xu Ning-yi 
  • Yan Qi-chuan 
Article

Abstract

Elaeagnus angustifolia Linn. has various ecological, medicinal and economical uses. An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to classify and analyse the intra-specific genetic relationships of seventeen populations of E. angustifolia, collected from the Xinjiang areas of China. Chromatograms of alcohol-soluble proteins produced by seventeen populations of E. angustifolia, were compared. Each chromatogram of alcohol-soluble proteins came from a single seed of one wild plant only. The results showed that when using a Waters Delta Pak. C18, 5 μm particle size reversed phase column (15 mm×3.9 mm), a linear gradient of 25%:_60% solvent B with flow rate of 1 ml/min and run time of 67 min, the chromatography yielded optimum separation of E. angustifolia alcohol-soluble proteins. Representative peaks in each population were chosen according to peak area and occurrence in every seed. The converted data on the elution peaks of each population were different and could be used to represent those populations. GSC (genetic similarity coefficients) of 41% to 62% showed a medium degree of genetic diversity among the populations in these eco-areas. Cluster analysis showed that the seventeen populations of E. angustifolia could be divided into six clusters at the GSC=0.535 level and indicated the general and unique biochemical markers of these clusters. We suggest that E. angustifolia distribution in these eco-areas could be classified into six variable species. RP-HPLC was shown to be a rapid, repeatable and reliable method for E. angustifolia classification and identification and for analysis of genetic diversity.

Key words

E. angustifolia Intra-specific genetic relationship Genetic diversity Biochemical marker 

CLC number

Q94 

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Copyright information

© Zhejiang University 2006

Authors and Affiliations

  • Wang Qiang 
    • 1
  • Ruan Xiao 
    • 1
  • Huang Jun-hua 
    • 2
  • Xu Ning-yi 
    • 1
  • Yan Qi-chuan 
    • 3
  1. 1.Ningbo Institute of TechnologyZhejiang UniversityNingboChina
  2. 2.School of ForestryXinjiang Agriculture UniversityUrumqiChina
  3. 3.School of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina

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