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Journal of Zhejiang University SCIENCE B

, Volume 7, Issue 3, pp 228–234 | Cite as

Effects of combination of irbesartan and perindopril on calcineurin expression and sarcoplasmic reticulum Ca2+-ATPase activity in rat cardiac pressure-overload hypertrophy

  • Jiang Qing-jun 
  • Xu Geng 
  • Mao Fei-fei 
  • Zhu You-fa 
Article

Abstract

Aim: To observe effects of angiotensin (Ang) II receptor antagonist (AT1) irbesartan and angiotensin-converting enzyme (ACE) inhibitor perindopril on rat myocardium calcineurin expression and sarcoplasmic reticulum Ca2+-ATPase activity in the model of pressure-overload cardiac hypertrophy. Methods: Forty male adult Sprague Dawley rats were divided into 5 groups. One group was treated by sham operation; four groups were myocardium hypertrophy cases caused by banding aortic above renal artery. Drugs were given one week after operation. Group 1: sham group, rats (n=8) were gavaged with normal saline 2 ml/(kg·d) (ig); Group 2: control group, rats (n=8) were treated with normal saline 2 ml/(kg·d) (ig); Group 3: rats (n=8) were given perindopril 2 mg/(kg·d) (ig); Group 4: rats (n=8) were treated with irbesartan 20 mg/(kg·d) (ig); Group 5: rats (n=8) were given irbesartan 20 mg/(kg·d) plus perindopril 2 mg/(kg·d) (ig). Morphometric determination, calcineurin expression and sarcoplasmic reticulum Ca2+-ATPase activity were done at the end of 6 week of drug intervention. Expression of calcineurin in myocardium was detected by immunohistochemistry. Results: Left ventricular mass index (LVMI), transverse diameter of myocardial cell (TDM), calcineurin activity were remarkably decreased after drug intervention and this decrease was most remarkable in the combination drug therapy group. Sarcoplasmic reticulum Ca2+-ATPase activity was increased after drug intervention, especially in the combined drug therapy group. Calcineurin expression in myocardium was remarkably decreased after drug intervention. LVMI was positively correlated with TDM and calcineurin, negatively correlated with sarcoplasmic reticulum Ca2+-ATPase. Conclusion: These data suggest that irbesartan and perindopril inhibit cardiac hypertrophy through the increased activity of sarcoplasmic reticulum Ca2+-ATPase and decreased expression of calcineurin. Their combination had better effects on regressing of ventricular hypertrophy.

Key words

Angiotensin (Ang) II receptor antagonist Angiotensin-converting enzyme inhibitor Calcineurin Sarcoplasmic reticulum Ca2+-ATPase Pressure overload Cardiac hypertrophy Rat 

CLC number

R363 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Jiang Qing-jun 
    • 1
    • 2
  • Xu Geng 
    • 1
  • Mao Fei-fei 
    • 3
  • Zhu You-fa 
    • 4
  1. 1.Department of Cardiology, the Second Affiliated Hospital, School of MedicineZhejiang UniversityHangzhouChina
  2. 2.Department of CardiologyNingbo Lihuili HospitalNingboChina
  3. 3.Department of Pharmacy, Children’s Hospital, School of MedicineZhejiang UniversityHangzhouChina
  4. 4.Department of Pathology, School of MedicineZhejiang UniversityHangzhouChina

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