Journal of Zhejiang University SCIENCE B

, Volume 6, Issue 12, pp 1170–1175 | Cite as

Evaluation of ST13 gene expression in colorectal cancer patients

  • Dong Qing-hua  (董庆华)
  • Zheng Shu  (郑树)
  • Hu Yue  (胡跃)
  • Chen Gong-xing  (陈功星)
  • Ding Jia-yi  (丁佳逸)
Article

Abstract

We identified a novel gene ST13 from a subtractive cDNA library of normal intestinal mucosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa (P<0.05). But there were no significant differences in the expression of ST13 as compared with different Dukes’ stage, tumor differentiation grade, invasion depth, lymph node metastasis and disease-specific survival.

Key words

ST13 Colorectal cancer Real-time PCR 

CLC number

R735 

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Copyright information

© Zhejiang University 2006

Authors and Affiliations

  • Dong Qing-hua  (董庆华)
    • 1
  • Zheng Shu  (郑树)
    • 1
  • Hu Yue  (胡跃)
    • 1
  • Chen Gong-xing  (陈功星)
    • 1
  • Ding Jia-yi  (丁佳逸)
    • 1
  1. 1.Cancer Institute, Second Affiliated Hospital, School of MedicineZhejiang UniversityHangzhouChina

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