Characterization and RNA2 nucleotide sequence of broad bean wilt virus 2 isolate P158
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Broad bean wilt virus 2 (BBWV2) isolate P158 was characterized and the complete nucleotide sequence of viral genomic RNA2 was determined. P158 has 30 nm diameter isometric particles. Double immunodiffusion test suggested that P158 has high antigenicity homology with BBWV2 isolate B935. SDS-PAGE result showed that the coat protein of P158 was comprised of two types of polypeptide with molecular weight of 44.7 kD and 21.9 kD, respectively. The genome of P158 was made up of two RNA molecules with the length of 6.0 kb and 3.6 kb, respectively. cDNA of RNA2 was cloned by RT-PCR and sequenced. RNA2 was composed of 3597 nucleotide (nt) residues excluding the poly(A) tail and contained single long open reading frame extending from nt 230 to nt 3424 in the viral sense RNA, and encoded a polyprotein ofMr 119002 (119K). Comparison of the polyprotein and the counterpart of isolate B935 indicated that the polyprotein was cleaved at Q/G (465/466) and Q/A (867/868), to release three mature proteins: a protein of unknown function, large and small subunit. Sequence comparisons of P158 with fabaviruses showed P158 had very high sequence homology with BBWV2 isolates and patchouli mild mosaic virus, but to a less extent with BBWV1 isolates.
Key wordsbroad bean wilt virus characterization RNA2 sequence
CLC numberTU311.3 TU973
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- Murphy, F. A., Fauquet, C. M., Bishop, D. H. L. et al., 1995. Virus Taxonomy-Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses. Springer-Verlag, Vienna, p. 341–347.Google Scholar
- Qi, Y. J., Zhou, X. P., Xue, C. Y., et al., 2000b. Nucleotide sequence of RNA2 and polyprotein processing sites of a Chinese isolate of broad bean wilt virus 2.Prog. in Nat. Sci.,10(9): 680–686.Google Scholar
- Sambrook, J., Fritsch, E. F. and Maniatis, T., 1989. Molecular cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, New York, p. 1063.Google Scholar
- Zhou, X. P., Qian, X. H., Zhang, L. C., et al., Isolation and identification of broad bean wilt virus in faba bean.Journal of Zhejiang University (Natural Science),28 (supplement): 271–277.Google Scholar
- Zhou, X. P. and Li, D. B., 1996. Purification of broad bean wilt fabavirus and analysis on the components of virus proteins and genomic RNAs.Chinese Journal of Virology,12(4): 364–370.Google Scholar