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Journal of Zhejiang University-SCIENCE B

, Volume 19, Issue 8, pp 654–661 | Cite as

Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2

  • Guang-yong Ye
  • Ke-yi Wang
  • Qiao-di Gui
  • Min Wang
Article
  • 7 Downloads

Abstract

Objective

The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipidassociated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs).

Methods

LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry.

Results

LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA).

Conclusions

LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.

Key words

Ureaplasma urealyticum Lipid-associated membrane protein Human amniotic epithelial cell Toll-like receptor 2 

解脲脲原体脂质相关膜蛋白经Toll 样受体2 信号通路调控人羊膜上皮细胞诱导IL-6、IL-8 和TNF-α 的产生

中文概要

目的

探讨解脲脲原体(Ureaplasma urealyticum)及其脂质相关膜蛋白(LAMPs)作用于人羊膜上皮细胞(HAECs)过程中白介素6(IL-6)、IL-8 和肿瘤坏死因子α(TNF-α)的变化情况,阐明Toll样受体2(TLR2)的调控机制,明确解脲脲原体潜在的致病性。

创新点

从解脲脲原体诱导炎症反应的分子机制入手,提出TLR2 信号通路在其中的关键作用。

方法

经TX-114 处理萃取解脲脲原体获得LAMPs,将LAMPs 和解脲脲原体分别感染HAECs,用酶联免疫吸附试验(ELISA)测定炎症细胞因子(IL-6、IL-8 和TNF-α);采用实时聚合酶链反应(real-time PCR)测定TLR2 mRNA 水平,用蛋白质印迹(Western blot)检测TLR2 的表达量;经TLR2 阻断剂(anti-hTLR2-IgA)处理后,测定相应炎症细胞因子。

结论

解脲脲原体LAMPs 能诱导HAECs 的TLR2 表达上调和炎症因子增加,从而发生炎症反应;TLR2受阻断后,炎症因子表达减少,炎症水平下降。TLR2 在解脲脲原体LAMPs 感染HAECs 过程起关键作用。

关键词

解脲脲原体;脂质相关膜蛋白;人羊膜上皮细胞;Toll 样受体2

关键词

解脲脲原体 脂质相关膜蛋白 人羊膜上皮细胞 Toll 样受体2 

CLC number

R375+.3 

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Copyright information

© Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Women’s HospitalZhejiang University School of MedicineHangzhouChina
  2. 2.Central LaboratoryHangzhou First People’s HospitalHangzhouChina
  3. 3.Department of Clinical LaboratoryShaanxi Provincial People’s HospitalXi’anChina

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