The objective of the present in vitro research was to determine cardiomyocyte functions on poly-lactic-co-glycolic acid (50:50 (PLA:PGA); PLGA) with greater amounts of carbon nanofibers (CNFs) using an in vitro electrical stimulation system for myocardial tissue engineering applications. The addition of CNFs can increase the conductivity and strength of pure PLGA. For this reason, different PLGA: CNF ratios (100:0, 75:25, 50:50, 25:75, 0:100 wt%) were created where conductivity and cytocompatibility properties under electrical stimulation with human cardiomyocytes were determined. Results showed that PLGA:CNF materials were conductive and that conductivity increased with greater amounts of PLGA added, from 0 S.m−1 for 100:0 wt% (pure PLGA) to 6.5×10−3 S.m−1 for 0:100 wt% (pure CNFs) materials. Furthermore, results indicated that cardiomyocyte cell density increased with continuous electrical stimulation (rectangular, 2 nm, 5 V/cm, 1 Hz) after 1, 3, and 5 days as well as a slight increase in Troponin I excretion compared to non-electrically stimulated normal cardiomyocyte cell functions. This study, thus, provides an alternative conductive scaffold using nanotechnology which should be further explored for numerous cardiovascular applications.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Alpert J.S. and K. Thygesen, A Call for Universal Definitions in Cardiovascular Disease. Circulation, 2006. 114(8): p. 757–758.
Maton A., Human biology and health 1993, Englewood Cliffs, N.J.: Prentice Hall.
Members W.G., Executive Summary: Heart Disease and Stroke Statistics—2010 Update. Circulation, 2010. 121(7): p. 948–954.
Stout D.A., B. Basu, and T.J. Webster, Poly(lactic–co-glycolic acid): Carbon nanofiber composites for myocardial tissue engineering applications. Acta Biomaterialia, 2011. 7(8): p. 3101–3112.
Pedrotty D.M., J. Koh, B. H. Davis, D. A. Taylor, P. Wolf & L. E. Niklason. Engineering skeletal myoblasts: roles of three-dimensional culture and electrical stimulation. Am J Physiol Heart Circ Physiol, 2005. 288(4): p. H1620–1626.
Yang L., B.W. Sheldon, and T.J. Webster, The impact of diamond nanocrystallinity on osteoblast functions. Biomaterials, 2009. 30(20): p. 3458–3465.
Mihardja S.S., R.E. Sievers, and R.J. Lee, The effect of polypyrrole on arteriogenesis in an acute rat infarct model. Biomaterials, 2008. 29(31): p. 4205–4210.
The authors would like to thank the National Science Foundation Graduate Research Fellowship Program (NSF #1058262) and the Hermann Foundation for funding and support.
About this article
Cite this article
Stout, D.A., Raimondo, E. & Webster, T.J. Improved Cardiomyocyte Functions of Carbon Nanofiber Cardiac Patches. MRS Online Proceedings Library 1417, 763 (2012). https://doi.org/10.1557/opl.2012.763