Differences in Nanoscale Elasticity of Planar and Nanofibrillar Tissue Cultures


Astrocytes are cellular bridges between the neurons and capillaries in the blood brain barrier. It was recently suggested that the nanophysical properties of the basement membrane of the blood brain barrier can influence astrocyte and neuron responses. In this work, cerebral cortical astrocytes were cultured on standard poly-L-Lysine coated glass substrates, Aclar substrates, and electrospun polyamide nanofibers whose properties may recapitulate those of the basement membrane. The nanoscale elasticity of each culture environment was investigated by force curve analysis and compared. The elasticity of the individual nanofibers on nanofibrillar surfaces was also investigated. Finally, variations in elasticity of scaffolds were correlated with astrocyte responses.

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  1. [1]

    D.E. Discher, P. Jamney and Y. Wang, Science 310, 1139, (2005).

    CAS  Article  Google Scholar 

  2. [2]

    S.-Y. Tee, J. Fu, C. S. Chen and P. A. Janmey, Biophys. J. 100, 25, (2011).

    Article  Google Scholar 

  3. [3]

    D.C. Lin, E.K. Dimitriadis and F. Horkay, Transactions of the ASME 129, (2007).

  4. [4]

    W.F. Heinz and J.H. Hoh, TIBTECH, 17, 143, (1999).

    CAS  Article  Google Scholar 

  5. [5]

    S. Meiners, I. Ahmed, A.S. Ponery, N. Amor, V.M. Ayres, Y. Fan, Q. Chen and A.N. Babu, Polymer Int., 56, 1340, (2007).

    CAS  Article  Google Scholar 

  6. [6]

    R. Delgado-Rivera, S.L. Harris, I. Ahmed, A.N. Babu, R. Patel, V.M. Ayres, D.A. Flowers and S. Meiners, Matrix Bio. 28, 137, (2009).

    CAS  Article  Google Scholar 

  7. [7]

    V. Caciuc, H. Hölscher, S Blügel and H Fuchs, Nanotechnology, 16, S59, (2005).

    CAS  Article  Google Scholar 

  8. [8]

    H.J. Butt, B. Cappella and M. Kappl, Surf. Sci. Rep., 59, 1, (2005).

    CAS  Article  Google Scholar 

  9. [9]

    C.S. Lee, E. Jones, and R. Kingsland, Advances in Polymer Technology, 6, 85, (1986).

    CAS  Article  Google Scholar 

  10. [10]

    A. Nur-e-Kamal, I. Ahmed, J. Kamal, M. Schindler and S. Meiners, Biochem. Biophys. Res. Commun. 331, 428, (2005).

    CAS  Article  Google Scholar 

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Correspondence to Volkan Mujdat Tiryaki.

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Tiryaki, V.M., Ayres, V.M., Ahmed, I. et al. Differences in Nanoscale Elasticity of Planar and Nanofibrillar Tissue Cultures. MRS Online Proceedings Library 1417, 746 (2012). https://doi.org/10.1557/opl.2012.746

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