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Anti-Tumor Chloroquine-Gold Nanocomposites and their Binding Interaction with Bovine Serum Albumin: Biophysical and Biochemical Aspects of Protein Binding

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Abstract

We have conjugated chloroquine onto nano-sized, thiol-stabilized gold nanoparticles by using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) / N-hydroxysulfosuccinimide (NHS) chemistry. The formation of gold nanoparticles was confirmed using optical spectra for characteristic surface plasmon band; the average size of gold nanoparticles was found to be 5-7 nm from electron microscopy measurements. The anti-tumor activity of prepared nanocomposite, vis-à-vis chloroquine itself, had been demonstrated using MCF-7 breast cancer cell line. To determine the binding affinity of gold-chloroquine nanocomposites to transport proteins present in blood serum, we studied the binding interaction of gold-chloroquine to bovine serum albumin (BSA), the most abundant plasma protein. The binding was studied by using isothermal titration calorimetry and fluorescence spectroscopy and was analyzed in terms of binding constant, entropy and enthalpy change. The gold-chloroquine nanocomposites were found to interact efficiently with BSA and fluorescence quenching experiments involving Trp212 suggests that the nanocomposites bind at site I of BSA.

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Authors and Affiliations

  1. National Physical Laboratory, Dr. K. S. Krishnan Marg, 110012, New Delhi, India

    Prachi Joshi, Virendra Shanker & P. Singh Surinder

  2. Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, 110025, New Delhi, India

    Prachi Joshi & A. Z. Ansari

  3. Department of Biochemistry, Bose Institute, P-1/12 CIT Scheme VIIM, 700054, Kolkata, India

    Soumyananda Chakraborti & Pinak Chakrabarti

  4. Engineering Science and Materials Department, University of Puerto Rico, 00680, Mayaguez, PR, USA

    E. Ramirez-Vick Jaime & P. Singh Surinder

Authors
  1. Prachi Joshi
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  2. Soumyananda Chakraborti
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  3. E. Ramirez-Vick Jaime
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  4. A. Z. Ansari
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  5. Virendra Shanker
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  6. Pinak Chakrabarti
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  7. P. Singh Surinder
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Correspondence to Pinak Chakrabarti.

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Joshi, P., Chakraborti, S., Jaime, E.RV. et al. Anti-Tumor Chloroquine-Gold Nanocomposites and their Binding Interaction with Bovine Serum Albumin: Biophysical and Biochemical Aspects of Protein Binding. MRS Online Proceedings Library 1316, 929 (2011). https://doi.org/10.1557/opl.2011.595

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  • DOI: https://doi.org/10.1557/opl.2011.595

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