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Label-free Electrochemical Biosensor Based on Graphene/Ionic Liquid Nanocomposite for the Detection of Organophosphate Pesticides

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Abstract

The advanced electrodes for detecting organophosphate pesticides were prepared by modification of the gold (Au) electrode with the reduced graphene oxide/ionic liquid (RGO/IL) nanohybrids. Due to the cationic and anionic parts, the ILs on RGO sheets provide the amount of functional groups for dispersion of hybrids and immobilization of organophosphorus hydrolase (OPH) enzymes. After the immobilization of OPH on the RGO/IL-modified Au electrodes, the modified electrodes represent faster electron transfer than that of Au electrode, resulting in high performance of biosensor with response time (~ 10 s) and sensitivity (4.56 nA μM−1). In addition, the OPH/RGO/IL-modified Au electrode displayed good stability and reproducibility.

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Authors and Affiliations

  1. BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, 305-701, Republic of Korea

    Tae Jung Park, Nam Su Heo & Sang Yup Lee

  2. Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon, 305-701, Republic of Korea

    MinHo Yang, Bong Gill Choi, Won Hi Hong & Sang Yup Lee

  3. NTD&I Team, National Nanofab Center, Daejeon, Republic of Korea

    Seok Jae Lee

  4. Department of Bio & Brain Engineering, Department of Biological Sciences, and Bioinformatics Research Center, KAIST, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee

Authors
  1. Tae Jung Park
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  2. MinHo Yang
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  3. Bong Gill Choi
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  4. Nam Su Heo
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  5. Seok Jae Lee
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  6. Won Hi Hong
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  7. Sang Yup Lee
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Park, T.J., Yang, M., Choi, B.G. et al. Label-free Electrochemical Biosensor Based on Graphene/Ionic Liquid Nanocomposite for the Detection of Organophosphate Pesticides. MRS Online Proceedings Library 1283, 805 (2010). https://doi.org/10.1557/opl.2011.325

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  • DOI: https://doi.org/10.1557/opl.2011.325

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