200 mm wafer-scale fabrication of polydimethylsiloxane fluidic devices for fluorescence imaging of single DNA molecules

Abstract

We report fabrication of 200 mm silicon (Si)-wafer mold structure for polydimethylsiloxane (PDMS) microfluidic devices to demonstrate a real-time fluorescence imaging of single DNA molecules. Conventional photolithography with deep reactive ion etching process allows us to build a “mesa”-type Si mold with a nanoscallop sidewall geometry aiding PDMS residue-free process. By optimizing fluorescence microscopy with the fabricated PDMS chamber, we obtain a protocol to visualize the motions of single DNA molecules. This integrative PDMS-based single-molecule imaging system can, in principle, be used as a platform to study biochemical reactions occurring in proteins, nucleotides, and vesicles.

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ACKNOWLEDGMENTS

This work was supported by KNU research fund 2016.

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Correspondence to Sung-Wook Nam.

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The supplementary material for this article can be found at https://doi.org/10.1557/mrc.2018.58

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Nam, SW. 200 mm wafer-scale fabrication of polydimethylsiloxane fluidic devices for fluorescence imaging of single DNA molecules. MRS Communications 8, 420–427 (2018). https://doi.org/10.1557/mrc.2018.58

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