Bio-inspired synthesis of superparamagnetic iron oxide nanoparticles for enhanced in vitro anticancer therapy

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONPs) are successfully synthesized in this study by co-precipitation method using actinobacterial metabolites as reducing agent. Physicochemical and morphological features of the nanoparticles (NPs) are analyzed by Fourier-transform infrared spectroscopy, x-ray-based techniques, vibrating sample magnetometer, thermal gravimetric analysis, and electron microscopic analysis, with an average size of 15-30 nm. Anticancer activity of the magnetite-NPs is systematically evaluated on HeLa cells using MTT assay, Hoechst nuclear staining, acridine orange/ethidium bromide dual staining and flow cytometric analysis. The obtained results open a new route for biosynthesis of SPIONPs, which to be used for various biomedical applications, particularly in cancer therapy.

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Acknowledgment

One ofthe authors, T. Shanmugasundaram wishes to thankthe SERB, India for financial support in the form of National Post-Doctoral Fellowship (PDF/2016/003119).

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Correspondence to Ramasamy Balagurunathan.

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Shanmugasundaram, T., Radhakrishnan, M., Poongodi, A. et al. Bio-inspired synthesis of superparamagnetic iron oxide nanoparticles for enhanced in vitro anticancer therapy. MRS Communications 8, 604–609 (2018). https://doi.org/10.1557/mrc.2018.36

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