The objective of the present work was to evaluate the behavior of osteogenesis of mesenchymal stem cells (MSCs) on a double-layer, protective, and bioactive hybrid coating sterilized by 3 different processes: steam autoclave, hydrogen peroxide plasma, and ethylene oxide. The hybrid coating was obtained from a sol consisting of the silane precursors tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES), applied on a Ti6Al4V substrate. To promote bioactivity, hydroxyapatite (HA) particles were dispersed in a second coating (bioactive layer: TEOS/MTES + HA) applied on the first (TEOS/MTES). The sterilized coatings were evaluated by scanning electron microscopy, wettability, and micrometer roughness. The behavior of hydrolytic degradation was evaluated by the mass variation of the samples and the release of silicon by the technique of high-resolution atomic absorption spectrometry. All coatings presented morphological and superficial alterations after sterilization. Sterilization by ethylene oxide and hydrogen peroxide plasma intensified the hydrolytic degradation of the bioactive coating causing a greater release of silicon. The sterilized hybrid coatings did not show cytotoxicity to MSCs. Adhesion, viability, and osteogenic differentiation were favored on the sterilized coating of hydrogen peroxide plasma, which is opposite to what was observed for the ethylene oxide-sterilized coating.
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The present work was developed with the support of the Brazilian government through the National Council of Scientific and Technological Development CNPq (CNPq/PVE 401211/2014-2 and CNPq 308773/2014-4) and the Coordination of Improvement of Higher Education Personnel (Capes). The authors would also like to thank the company Esterilizare RS/Brazil and Hospital Pompéia RS/Brazil, for their support in carrying out the processes and sterilization.
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Baldin, E.K.K., de Fraga Malfatti, C., Brandalise, R.N. et al. Osteogenic differentiation of mesenchymal stem cells on hybrid coatings sterilized by different processes. Journal of Materials Research 34, 3400–3411 (2019). https://doi.org/10.1557/jmr.2019.291