The hydroxyapatite nanoparticles (nHAPs) were synthesized rapidly by the self-assembled dual-frequency ultrasonic method. The ultrasonic time and power effect on the morphology and phase composition of nHAPs were investigated through field-emission scanning electron microscopy (FE-SEM), X-ray diffraction, energy dispersive spectrometer (EDS) spectrometer, and Fourier transform infrared spectroscopy, which showed that the most uniform nanoparticles were obtained when the ultrasonic time was 30 min and the ultrasonic power was 280 W. Cytotoxicity and hemolysis tests showed that an indistinctive cytotoxic effect was within the concentration of 25–400 μg/mL and the hemolytic ratio was below 2.0% at concentration of 25–200 μg/mL, respectively, revealing a good biocompatibility of nHAPs. By loading tetracycline hydrochloride onto nHAPs spheres, the drug release results showed that the drug loading and encapsulation efficiency were (26.34 ± 2.99)% and (52.68 ± 5.98)%, respectively. The drug-loaded sample shows a slow-release property, indicating that nHAPs may be promising as drug carriers.
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This work was supported by the Open Project Program of The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China (No. 201701), Research and Demonstration on the Key Technology of Drinking Water and Sewage Treatment in the Northwest Desertification Area (No. 2016YFC0400707), Guangdong Provincial Department of Water Resources Project (No. 2015-20), and Guangdong Provincial Archives Project (No. YDK-141-2016).
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Deng, St., Lin, Zt., Tang, Hx. et al. Rapid synthesis of hydroxyapatite nanoparticles via a novel approach in the dual-frequency ultrasonic system for specific biomedical application. Journal of Materials Research 34, 2796–2806 (2019). https://doi.org/10.1557/jmr.2019.119