Chronic osteomyelitis, a bone infection caused by bacteria, requires extensive parenteral treatments. With an aim to develop bioactive glass with antibacterial properties to resist such infections, bioactive glasses with bismuth oxide as the dopant in various amounts up to 8 wt% were prepared. X-ray diffraction patterns and Fourier-transform infrared spectra of glass samples after immersion in simulated body fluid showed the presence of hydroxyapatite (HAp) and hydroxyl carbonate apatite for all samples except with the one having Bi2O3 substitution of 8 wt%. In vitro cell proliferation by MTT assay studies using a mouse fibroblast cell line (NIH3T3) have also been carried out. Primary antimicrobial activity of the glass particles was analyzed against Escherichia coli (E. coli) using broth microdilution method which exhibited bacteriostatic effects and bactericidal properties in selected samples. The combination of bioactivity, cell proliferation, and antibacterial properties of selected Bismuth-containing bioactive glasses could be exploited in treating bone-related infections.
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Authors would like to express their grateful thanks to Dr. K. Muraleedharan, Director, CSIR-CGCRI and Dr. Ranjan Sen, Head, Glass division for their encouragement and support toward this work. The financial help from P 81-113 of the CSIR Extramural Research Fund for Young Scientists and CSIR-HRDG (Extramural Research Group) for providing GATE-JRF fellowship to SPS for pursuing Integrated M.Tech Ph.D. program is gratefully acknowledged. The authors gratefully acknowledge the suggestions and experimental help offered by Dr. Biswanath Kundu, Senior Scientist and Dr. Vamsi K. Balla, Senior Principal Scientist, Bioceramics and Coating Division, CSIR-CGCRI.
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Prasad S, S., Ratha, I., Adarsh, T. et al. In vitro bioactivity and antibacterial properties of bismuth oxide modified bioactive glasses. Journal of Materials Research 33, 178–190 (2018). https://doi.org/10.1557/jmr.2017.442