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Electrochemical detection of non-labeling DNA using electronic array

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Abstract

Our system is the electrochemical approaches include the detection of hybridization from nonlabeling nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In order to generate bio-functional surfaces, the streptavidin(SAv)-biotin system is used. A 50 % change of redox peak current after hybridization measured with 50 ?M concentration of target DNA. We suggest that this result comes from the efficient electron transport through the SAv-biotin interaction. Our electrochemical detection system showed good reproducibility on a chip with non-labeling DNA hybridization detection.

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References

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Authors and Affiliations

  1. The Institute of Scientific and Industrial Research, Osaka University, 8–1 Mihogaoka, 567-0047, Ibaraki, Osaka, Japan

    H. Y. Lee, J. W. Park, Y. S. Choi, T. Kannno, Hiro Tanaka, Hide Tanaka & T. Kawai

Authors
  1. H. Y. Lee
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  2. J. W. Park
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  3. Y. S. Choi
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  4. T. Kannno
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  5. Hiro Tanaka
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  6. Hide Tanaka
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  7. T. Kawai
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Corresponding author

Correspondence to T. Kawai.

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Lee, H.Y., Park, J.W., Choi, Y.S. et al. Electrochemical detection of non-labeling DNA using electronic array. MRS Online Proceedings Library 761, 1616 (2002). https://doi.org/10.1557/PROC-761-NN1.6/C1.6

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  • DOI: https://doi.org/10.1557/PROC-761-NN1.6/C1.6

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