Our system is the electrochemical approaches include the detection of hybridization from nonlabeling nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In order to generate bio-functional surfaces, the streptavidin(SAv)-biotin system is used. A 50 % change of redox peak current after hybridization measured with 50 ?M concentration of target DNA. We suggest that this result comes from the efficient electron transport through the SAv-biotin interaction. Our electrochemical detection system showed good reproducibility on a chip with non-labeling DNA hybridization detection.
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Lee, H.Y., Park, J.W., Choi, Y.S. et al. Electrochemical detection of non-labeling DNA using electronic array. MRS Online Proceedings Library 761, 1616 (2002). https://doi.org/10.1557/PROC-761-NN1.6/C1.6