Electrochemical detection of non-labeling DNA using electronic array

Abstract

Our system is the electrochemical approaches include the detection of hybridization from nonlabeling nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In order to generate bio-functional surfaces, the streptavidin(SAv)-biotin system is used. A 50 % change of redox peak current after hybridization measured with 50 ?M concentration of target DNA. We suggest that this result comes from the efficient electron transport through the SAv-biotin interaction. Our electrochemical detection system showed good reproducibility on a chip with non-labeling DNA hybridization detection.

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References

  1. 1.

    T. Kawai, Proc. µTAS2002, 1, 43 (2002)

    CAS  Google Scholar 

  2. 2.

    Louis Tiefenauer, et al. Biosensor & Biochemistry, 15, 431(2000)

    Google Scholar 

  3. 3.

    Ronald G. Sosnowski, et al. Proc. Natl.Acad.Sci., 94, 1119 (1997)

    CAS  Article  Google Scholar 

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Correspondence to T. Kawai.

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Lee, H.Y., Park, J.W., Choi, Y.S. et al. Electrochemical detection of non-labeling DNA using electronic array. MRS Online Proceedings Library 761, 1616 (2002). https://doi.org/10.1557/PROC-761-NN1.6/C1.6

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