Nanocrystalline diamond thin films of sub-micron thickness have been covalently modified with DNA oligonucleotides. Quantitative studies of hybridization of surface-bound oligonucleotides with fluorescently tagged complementary and non-complementary oligonucleotides were performed. The results show no detectable nonspecific adsorption, with extremely good selectivity between matched and mismatched sequences. Impedance spectroscopy measurements were made of DNA-modified boron-doped nanocrystalline diamond films. The results show that exposure to non-complementary sequences induce only small changes in impedance, while complementary DNA sequences produce a pronounced decrease in impedance. The combination of high stability, selectivity, and the ability to directly detect DNA hybridization via electrical means suggest that diamond may be an ideal substrate for continuously-monitoring biological sensors.
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This work was supported in part by the U.S. Office of Naval Research N00014-01-1-0654, the National Science Foundation CHE-0071385, the Wisconsin Alumni Research Foundation, and the National Institutes of Health Grant R01 EB00269, and the US Department of Energy, BES-Materials Sciences, under Contract W-31-109-ENG-38.
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Yang, W., Auciello, O., Butler, J.E. et al. Preparation and Electrochemical Characterization of DNA-modified Nanocrystalline Diamond Films. MRS Online Proceedings Library 737, 44 (2002). https://doi.org/10.1557/PROC-737-F4.4