Comparative Study Of The Growth Curves Of B. subtilis, K. pneumoniae, C. xerosis And E. coli Bacteria In Medium Containing Nanometric Silicon Particles


In this research nanometric particles from luminescent (625nm) porous silicon film were synthesized. This particles were later inoculated in bacterial strains of B. subtilis (BSi) and K. pneumoniae (KSi). A comparison of the behavior of their growth curve and the ones reported for C. xerosis (XSi) and E. coli (ESi) in presence of silicon nanoparticles is presented. The growth curve of BSi, as well as the KSi, present changes compared to their standard curves. The BSi growth curve grows below the standard curve after the fifth hour, while in the KSi this happens after the eighth hour. Based on our preliminary findings we can speculate that at this point in time a critical population is present, and this may give rise to the possible incorporation of the silicon particles by the bacteria, or a possible pleomorphism inhibits reproduction. The stationary region, in both cases, takes place sooner than in the standard curve. No significant oscillations are observed in any case, which differs form the XSi curve, were oscillations of intervals of almost 1 hour were reported. In addition, these curves have a different behavior when compared to the ESi growth curve, in which no significant differences between the standard and the particle containing sample were reported.

This is a preview of subscription content, access via your institution.


  1. 1.

    M. Chee {etet al.}; Science 274, 610 (1996).

    CAS  Article  Google Scholar 

  2. 2.

    E.M. Southern {etet al.}; Nucleic Acids Res. 22 1368 (1994).

    Article  Google Scholar 

  3. 3.

    J.A. Ferguson,T.C. Boles, C.P. Adams, D. R. Walt, NatureBiotechnol, l4, 1681 (1996).

    Google Scholar 

  4. 4.

    M. Schena, D. Shalon, R.W. Davis, P.O. Brown, Science, 270, 467 (1995).

    CAS  Article  Google Scholar 

  5. 5.

    S. M. Barnard and D.R. Walt, Science, 251, 927 (1991).

    CAS  Article  Google Scholar 

  6. 6.

    P. S. Stayton {etet al.}, Nature, 378, 472 (1995).

    CAS  Article  Google Scholar 

  7. 7.

    F.J. Rowell, Biochem. Soc. Trans., 19, 32 (1991).

    CAS  Article  Google Scholar 

  8. 8.

    A. Rikin, E. Katz, I. Wilner, A. Stocker, A.F. Buckmann, Nature, 376, 672 (1995).

    Article  Google Scholar 

  9. 9.

    R. V. Parthasarathy and C.R. Martin, Nature, 369, 298 (1994).

    CAS  Article  Google Scholar 

  10. 10.

    Y. Shinohara {etet al.}, J. Biochem., 117, 1076 (1995).

    CAS  Google Scholar 

  11. 11.

    J. Harper, M. Sailor, J. Anal. Chem., 68, 3713 (1996).

    CAS  Article  Google Scholar 

  12. 12.

    A. Janshoff, K. P. Dancil, C. Steinem, D.P. Greinier, V. Lin, C. Gurtner, K. Motesharei, M. Sailor, M.R. Ghadiri,. J. Am. Chem. Soc., 120, 12108 (1998).

    CAS  Article  Google Scholar 

  13. 13.

    J. Wei, J. Buriak, G. Siuzdak, Nature, 399, 243 (1999).

    CAS  Article  Google Scholar 

  14. 14.

    M. Wainwrigth, L.T. Canham, K. Al-Wajeeh and C.L. Reeves, Lett. In Appl. Microbiology, 29, 224 (1999).

    Article  Google Scholar 

Download references


The authors would like to express their appreciation to Dr. Javier Avalos for his continuous support and for the opportunity to work on this project, to Professor Raquel Lara and Maricely Hernandez, for sharing their knowledge and time, to Luis Fonseca, PhD. and Oscar Resto, PhD from the Solid State Laboratory, UPR, for their cooperation, and to the Research team at the Thin Films Laboratory at UMET for their patience and persistence. Finally they would like to thank Dr. Juan Arratia, MIE and NSF for their support.

Author information



Corresponding author

Correspondence to Lilyanna Pérez.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Pérez, L., Flores, M., Avalos, J. et al. Comparative Study Of The Growth Curves Of B. subtilis, K. pneumoniae, C. xerosis And E. coli Bacteria In Medium Containing Nanometric Silicon Particles. MRS Online Proceedings Library 737, 36 (2002).

Download citation